Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin

The demand for bioactive component from natural resources has been increased worldwide for pharmaceutical purpose. The aim of this study is to evaluate the antimicrobial activity of Citrus hystrix, Citrus microcarpa and Citrus aurantifolia of juice and peel plant against gram-negative bacteria using...

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Main Author: Nazarudin, Nazihah
Format: Student Project
Language:English
Published: 2018
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/34061/1/34061.pdf
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author Nazarudin, Nazihah
author_facet Nazarudin, Nazihah
author_sort Nazarudin, Nazihah
collection UITM
description The demand for bioactive component from natural resources has been increased worldwide for pharmaceutical purpose. The aim of this study is to evaluate the antimicrobial activity of Citrus hystrix, Citrus microcarpa and Citrus aurantifolia of juice and peel plant against gram-negative bacteria using disc diffusion method. Gram-negative bacteria such as E.coli, Salmonella spp and Shigella spp are common types of species that can cause foodborne illness. Overall Salmonella spp is very susceptible against all the Citrus microcarpa, Citrus hystrix and Citrus aurantifolia peel compared to Shigella spp and E.coli spp. The highest inhibition zone recorded among the Citrus peel was from Citrus hystrix with 18.67 mm against Salmonella spp while the lowest is from Citrus microcarpa with 9 mm against Shigella spp. Besides that, the result for Citrus juice shows that all Citrus hystrix, Citrus microcarpa and Citrus aurantifolia are very susceptible to E.coli compared to the Salmonella spp and Shigella spp. The highest inhibition zone recorded is from Citrus hystrix against E.coli with inhibition zone recorded are 16.67 mm while the lowest is comes from C. aurantifolia against Salmonella spp with 8.67 mm. Overall the ANOVA result for the E.coli, Salmonella spp and Shigella spp against Citrus peel of Citrus hystrix, Citrus microcarpa and positive control show there is significance difference. Next, Salmonella spp and Shigella spp against Citrus juice of Citrus hystrix, Citrus microcarpa, Citrus aurantifolia and positive control shown there is significance except for E.coli where there is no siginificance difference against all the Citrus hystrix, Citrus microcarpa, Citrus aurantifolia and positive control because the p values is higher than 0.05. As conclusion, Citrus hystrix, Citrus microcarpa and Citrus aurantifolia peel are very good against Salmonella spp while Citrus hystrix, Citrus microcarpa and Citrus aurantifolia juice is very good against E.coli spp.
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spelling oai:ir.uitm.edu.my:340612020-08-28T04:35:12Z https://ir.uitm.edu.my/id/eprint/34061/ Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin Nazarudin, Nazihah Laboratories. General works Bacteria The demand for bioactive component from natural resources has been increased worldwide for pharmaceutical purpose. The aim of this study is to evaluate the antimicrobial activity of Citrus hystrix, Citrus microcarpa and Citrus aurantifolia of juice and peel plant against gram-negative bacteria using disc diffusion method. Gram-negative bacteria such as E.coli, Salmonella spp and Shigella spp are common types of species that can cause foodborne illness. Overall Salmonella spp is very susceptible against all the Citrus microcarpa, Citrus hystrix and Citrus aurantifolia peel compared to Shigella spp and E.coli spp. The highest inhibition zone recorded among the Citrus peel was from Citrus hystrix with 18.67 mm against Salmonella spp while the lowest is from Citrus microcarpa with 9 mm against Shigella spp. Besides that, the result for Citrus juice shows that all Citrus hystrix, Citrus microcarpa and Citrus aurantifolia are very susceptible to E.coli compared to the Salmonella spp and Shigella spp. The highest inhibition zone recorded is from Citrus hystrix against E.coli with inhibition zone recorded are 16.67 mm while the lowest is comes from C. aurantifolia against Salmonella spp with 8.67 mm. Overall the ANOVA result for the E.coli, Salmonella spp and Shigella spp against Citrus peel of Citrus hystrix, Citrus microcarpa and positive control show there is significance difference. Next, Salmonella spp and Shigella spp against Citrus juice of Citrus hystrix, Citrus microcarpa, Citrus aurantifolia and positive control shown there is significance except for E.coli where there is no siginificance difference against all the Citrus hystrix, Citrus microcarpa, Citrus aurantifolia and positive control because the p values is higher than 0.05. As conclusion, Citrus hystrix, Citrus microcarpa and Citrus aurantifolia peel are very good against Salmonella spp while Citrus hystrix, Citrus microcarpa and Citrus aurantifolia juice is very good against E.coli spp. 2018 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/34061/1/34061.pdf Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin. (2018) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Bacteria
Nazarudin, Nazihah
Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin
title Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin
title_full Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin
title_fullStr Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin
title_full_unstemmed Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin
title_short Comparison of antimicrobial activity of citrus fruit in Malaysia against gram-negative bacteria / Nazihah Nazarudin
title_sort comparison of antimicrobial activity of citrus fruit in malaysia against gram negative bacteria nazihah nazarudin
topic Laboratories. General works
Bacteria
url https://ir.uitm.edu.my/id/eprint/34061/1/34061.pdf
work_keys_str_mv AT nazarudinnazihah comparisonofantimicrobialactivityofcitrusfruitinmalaysiaagainstgramnegativebacterianazihahnazarudin