Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail
The rats were widely diverse around the world. The identification of rat were difficult since most rats share common morphology characteristics. Thus, the identification through genetic material might help to identify the rat more accurately since the rat species were difficult to identify through t...
| Main Author: | |
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| Format: | Student Project |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/34413/1/34413.pdf |
| _version_ | 1796903821574668288 |
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| author | Wan Ismail, Wan Muhammad Imran |
| author_facet | Wan Ismail, Wan Muhammad Imran |
| author_sort | Wan Ismail, Wan Muhammad Imran |
| collection | UITM |
| description | The rats were widely diverse around the world. The identification of rat were difficult since most rats share common morphology characteristics. Thus, the identification through genetic material might help to identify the rat more accurately since the rat species were difficult to identify through the morphology characteristic. The aim of this study was to identify the unknown caught rats using phylogeny-based identification and further compared rat identification through morphological characteristics. Five rats’ samples were obtained from five different areas and the morphological characteristics of each sample were recorded to identify their species. The five rats’ tails were taken for DNA extractions by using high-salt method. The extracted DNA were amplified and sequenced for cytochrome oxidase 1 region. A total of 745 base pair were successfully sequenced for all samples and were aligned with 77 sequences retrieve from GenBank. Three phylogenetic trees were reconstructed Neighbor-Joining, Bayesian inferences and maximum likelihood method. Based on the morphological characteristics, the five samples were most likely identified as Rattus norgevicus for Rat 1, Rat 2 as Rattus tiomanicus, Rat 3 as Rattus andamanensis, Raat 4 as Rattus norvegicus and Rat 5 as Rattus exulans. However, further identification through phylogeny-based identification from the three phylogenetic tree shows that Rat 1 highly probably identified as Rattus norvegius, while Rat 2, Rat 3, Rat 4 and Rat 5 as Rattus kandianus and/or Rattus andamanensis. |
| first_indexed | 2024-03-06T02:25:57Z |
| format | Student Project |
| id | oai:ir.uitm.edu.my:34413 |
| institution | Universiti Teknologi MARA |
| language | English |
| last_indexed | 2024-03-06T02:25:57Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | oai:ir.uitm.edu.my:344132020-09-15T03:26:24Z https://ir.uitm.edu.my/id/eprint/34413/ Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail Wan Ismail, Wan Muhammad Imran Laboratories. General works Extraction (Chemistry) DNA. Deoxyribonucleic acids The rats were widely diverse around the world. The identification of rat were difficult since most rats share common morphology characteristics. Thus, the identification through genetic material might help to identify the rat more accurately since the rat species were difficult to identify through the morphology characteristic. The aim of this study was to identify the unknown caught rats using phylogeny-based identification and further compared rat identification through morphological characteristics. Five rats’ samples were obtained from five different areas and the morphological characteristics of each sample were recorded to identify their species. The five rats’ tails were taken for DNA extractions by using high-salt method. The extracted DNA were amplified and sequenced for cytochrome oxidase 1 region. A total of 745 base pair were successfully sequenced for all samples and were aligned with 77 sequences retrieve from GenBank. Three phylogenetic trees were reconstructed Neighbor-Joining, Bayesian inferences and maximum likelihood method. Based on the morphological characteristics, the five samples were most likely identified as Rattus norgevicus for Rat 1, Rat 2 as Rattus tiomanicus, Rat 3 as Rattus andamanensis, Raat 4 as Rattus norvegicus and Rat 5 as Rattus exulans. However, further identification through phylogeny-based identification from the three phylogenetic tree shows that Rat 1 highly probably identified as Rattus norvegius, while Rat 2, Rat 3, Rat 4 and Rat 5 as Rattus kandianus and/or Rattus andamanensis. 2018 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/34413/1/34413.pdf Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail. (2018) [Student Project] (Unpublished) |
| spellingShingle | Laboratories. General works Extraction (Chemistry) DNA. Deoxyribonucleic acids Wan Ismail, Wan Muhammad Imran Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail |
| title | Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail |
| title_full | Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail |
| title_fullStr | Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail |
| title_full_unstemmed | Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail |
| title_short | Phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment / Wan Muhammad Imran Wan Ismail |
| title_sort | phylogenetic analysis of caught rat by using cytochrome oxidase 1 fragment wan muhammad imran wan ismail |
| topic | Laboratories. General works Extraction (Chemistry) DNA. Deoxyribonucleic acids |
| url | https://ir.uitm.edu.my/id/eprint/34413/1/34413.pdf |
| work_keys_str_mv | AT wanismailwanmuhammadimran phylogeneticanalysisofcaughtratbyusingcytochromeoxidase1fragmentwanmuhammadimranwanismail |