Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser

Archidendron pauciflorum (Jering) is the traditional medical plant from the family of Leguminosae that is native in Southeast Asia. It has been used traditionally to treat a various range of ailments. Genetic improvement is vital in the development of plants in order to increase their yield and qua...

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Main Author: Mohd Naser, Nuur Hidayatulsyimah
Format: Student Project
Language:English
Published: 2016
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/34452/1/34452.pdf
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author Mohd Naser, Nuur Hidayatulsyimah
author_facet Mohd Naser, Nuur Hidayatulsyimah
author_sort Mohd Naser, Nuur Hidayatulsyimah
collection UITM
description Archidendron pauciflorum (Jering) is the traditional medical plant from the family of Leguminosae that is native in Southeast Asia. It has been used traditionally to treat a various range of ailments. Genetic improvement is vital in the development of plants in order to increase their yield and quality, however there is no local data on plant tissue culture of Archidendron pauciflorum available. A protocol for successful callus induction from the seed of Archidendron pauciflorum was developed by using the embryo and cotyledon. The explants were inoculated onto Murashige and Skoog (MS) media supplemented with different levels (l.0 - 5.0 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA) and naphthalene acetic acid (NAA). After 21 days, compact white callus was obtained from the embryo supplemented with 2 mg/L of 2,4-D and friable white callus from 5 mg/L of NAA and 1 mg/L of IAA. Overall results showed that the presence of 2,4-D is the most suitable auxin to induce the callus on the seed of Archidendron pauciflorum.
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spelling oai:ir.uitm.edu.my:344522020-09-28T01:53:49Z https://ir.uitm.edu.my/id/eprint/34452/ Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser Mohd Naser, Nuur Hidayatulsyimah Laboratories. General works Growth Archidendron pauciflorum (Jering) is the traditional medical plant from the family of Leguminosae that is native in Southeast Asia. It has been used traditionally to treat a various range of ailments. Genetic improvement is vital in the development of plants in order to increase their yield and quality, however there is no local data on plant tissue culture of Archidendron pauciflorum available. A protocol for successful callus induction from the seed of Archidendron pauciflorum was developed by using the embryo and cotyledon. The explants were inoculated onto Murashige and Skoog (MS) media supplemented with different levels (l.0 - 5.0 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA) and naphthalene acetic acid (NAA). After 21 days, compact white callus was obtained from the embryo supplemented with 2 mg/L of 2,4-D and friable white callus from 5 mg/L of NAA and 1 mg/L of IAA. Overall results showed that the presence of 2,4-D is the most suitable auxin to induce the callus on the seed of Archidendron pauciflorum. 2016 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/34452/1/34452.pdf Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser. (2016) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Growth
Mohd Naser, Nuur Hidayatulsyimah
Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser
title Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser
title_full Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser
title_fullStr Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser
title_full_unstemmed Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser
title_short Callus induction from the seed of archidendron pauciflorum (jering) / Nuur Hidayatulsyimah Mohd Naser
title_sort callus induction from the seed of archidendron pauciflorum jering nuur hidayatulsyimah mohd naser
topic Laboratories. General works
Growth
url https://ir.uitm.edu.my/id/eprint/34452/1/34452.pdf
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