Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim

This study was focused on the crude extraction of leaves Artocarpus altilis with three different solvents (methanol, chloroform and petroleum ether) by soxhlet extraction. The crude extracts was continued to investigate the TLC profiling, antioxidant activity via DPPH method and antibacterial activi...

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Main Author: Ibrahim, Nur Ezzati
Format: Student Project
Language:English
Published: 2017
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/35128/1/35128.pdf
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author Ibrahim, Nur Ezzati
author_facet Ibrahim, Nur Ezzati
author_sort Ibrahim, Nur Ezzati
collection UITM
description This study was focused on the crude extraction of leaves Artocarpus altilis with three different solvents (methanol, chloroform and petroleum ether) by soxhlet extraction. The crude extracts was continued to investigate the TLC profiling, antioxidant activity via DPPH method and antibacterial activity by disc diffusion method. Methanol extract gave the highest weight percentage, 6.44%, For TLC profiling, methanol extract showed spot by using hexane: ethyl acetate (4:1) solvent system while chloroform and PE showed the spot at petroleum ether: ethyl acetate (2:3) and chloroform: ethyl acetate (3.5:1.5) respectively. Methanol extract showed the highest percentage of DPPH scavenging at 1000 μg/mL which is 80.62% compare to both chloroform and petroleum ether extracts for antioxidant activity. Staphylococcus aureus, Bacillus subtilis, Salmonella sp and Escherichia coli were used to evaluate the antibacterial activity of leaves extracts. Chloroform extract showed the highest zone of inhibition which is 11 mm against both Bacillus subtilis and Salmonella sp. Methanol extract showed the highest inhibition, 9 mm against Escherichia coli but PE extract showed the lowest inhibition, 7 mm against Escherichia coli. All three extracts showed significant activities against the four tested bacteria.
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spelling oai:ir.uitm.edu.my:351282020-10-15T15:02:00Z https://ir.uitm.edu.my/id/eprint/35128/ Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim Ibrahim, Nur Ezzati Laboratories. General works Extraction (Chemistry) Antioxidants Antibacterial agents This study was focused on the crude extraction of leaves Artocarpus altilis with three different solvents (methanol, chloroform and petroleum ether) by soxhlet extraction. The crude extracts was continued to investigate the TLC profiling, antioxidant activity via DPPH method and antibacterial activity by disc diffusion method. Methanol extract gave the highest weight percentage, 6.44%, For TLC profiling, methanol extract showed spot by using hexane: ethyl acetate (4:1) solvent system while chloroform and PE showed the spot at petroleum ether: ethyl acetate (2:3) and chloroform: ethyl acetate (3.5:1.5) respectively. Methanol extract showed the highest percentage of DPPH scavenging at 1000 μg/mL which is 80.62% compare to both chloroform and petroleum ether extracts for antioxidant activity. Staphylococcus aureus, Bacillus subtilis, Salmonella sp and Escherichia coli were used to evaluate the antibacterial activity of leaves extracts. Chloroform extract showed the highest zone of inhibition which is 11 mm against both Bacillus subtilis and Salmonella sp. Methanol extract showed the highest inhibition, 9 mm against Escherichia coli but PE extract showed the lowest inhibition, 7 mm against Escherichia coli. All three extracts showed significant activities against the four tested bacteria. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/35128/1/35128.pdf Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim. (2017) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Extraction (Chemistry)
Antioxidants
Antibacterial agents
Ibrahim, Nur Ezzati
Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim
title Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim
title_full Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim
title_fullStr Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim
title_full_unstemmed Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim
title_short Study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis (breadfruits) / Nur Ezzati Ibrahim
title_sort study of antioxidant and antibacterial activities of leaves extracts from artocarpus altilis breadfruits nur ezzati ibrahim
topic Laboratories. General works
Extraction (Chemistry)
Antioxidants
Antibacterial agents
url https://ir.uitm.edu.my/id/eprint/35128/1/35128.pdf
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