Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun
Lipase from Candida rugosa was immobilised onto N-vinyl-2pyrrolidone- co-styrene (VP-co-ST) hydrogel. The ethylene dimethacrylate (EDMA) and azoisobutyronitrile (AIBN) were used as a cross-linking agent and polymerization promoter respectively. This particular VP-co-ST hydrogel immobilised lipas...
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| Format: | Article |
| Language: | English |
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Universiti Teknologi MARA Cawangan Pahang
2006
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| Online Access: | https://ir.uitm.edu.my/id/eprint/35569/1/35569.PDF |
| _version_ | 1796904044576374784 |
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| author | Harun, Aiza |
| author_facet | Harun, Aiza |
| author_sort | Harun, Aiza |
| collection | UITM |
| description | Lipase from Candida rugosa was immobilised onto N-vinyl-2pyrrolidone-
co-styrene (VP-co-ST) hydrogel. The ethylene
dimethacrylate (EDMA) and azoisobutyronitrile (AIBN) were used
as a cross-linking agent and polymerization promoter respectively.
This particular VP-co-ST hydrogel immobilised lipase was used to
catalyse the enantioselective esterification of (R,S) -2-(4chlorophenoxy)
propanoic acid with n-tetradecanol. Only VP-coST
hydrogel immobilised lipase with composition of VP 10% and ST
90% and native lipase (unimmobilised lipase) will be discussed in
this study. The reaction was performed at optimum reaction time of
24 hours and at optimum temperature 40"C in organic media. The
study revealed that water activity, a iv may influence the
enantioselective esterification in which at lower a value, the
percentage of ester conversion was greater compared with at higher
all' value when using both hydrogel immobilised lipase and native
lipase in the reaction. However, when comparing between these two
lipases, the hydrogel immobilised lipase exhibited higher percentage
of ester conversion rather than native lipase at optimum water activity
value of 0.3. |
| first_indexed | 2024-03-06T02:29:02Z |
| format | Article |
| id | oai:ir.uitm.edu.my:35569 |
| institution | Universiti Teknologi MARA |
| language | English |
| last_indexed | 2024-03-06T02:29:02Z |
| publishDate | 2006 |
| publisher | Universiti Teknologi MARA Cawangan Pahang |
| record_format | dspace |
| spelling | oai:ir.uitm.edu.my:355692021-02-18T04:31:31Z https://ir.uitm.edu.my/id/eprint/35569/ Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun gading Harun, Aiza Organic chemistry Biochemistry Lipase from Candida rugosa was immobilised onto N-vinyl-2pyrrolidone- co-styrene (VP-co-ST) hydrogel. The ethylene dimethacrylate (EDMA) and azoisobutyronitrile (AIBN) were used as a cross-linking agent and polymerization promoter respectively. This particular VP-co-ST hydrogel immobilised lipase was used to catalyse the enantioselective esterification of (R,S) -2-(4chlorophenoxy) propanoic acid with n-tetradecanol. Only VP-coST hydrogel immobilised lipase with composition of VP 10% and ST 90% and native lipase (unimmobilised lipase) will be discussed in this study. The reaction was performed at optimum reaction time of 24 hours and at optimum temperature 40"C in organic media. The study revealed that water activity, a iv may influence the enantioselective esterification in which at lower a value, the percentage of ester conversion was greater compared with at higher all' value when using both hydrogel immobilised lipase and native lipase in the reaction. However, when comparing between these two lipases, the hydrogel immobilised lipase exhibited higher percentage of ester conversion rather than native lipase at optimum water activity value of 0.3. Universiti Teknologi MARA Cawangan Pahang 2006-10 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/35569/1/35569.PDF Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun. (2006) Jurnal Gading UiTM Pahang <https://ir.uitm.edu.my/view/publication/Jurnal_Gading_UiTM_Pahang/>, 10 (1). ISSN 0128-5599 |
| spellingShingle | Organic chemistry Biochemistry Harun, Aiza Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun |
| title | Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun |
| title_full | Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun |
| title_fullStr | Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun |
| title_full_unstemmed | Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun |
| title_short | Influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa / Aiza Harun |
| title_sort | influence of water activity on the enantioselective esterification catalysed by hydrogel immobilised lipase from candida rugosa aiza harun |
| topic | Organic chemistry Biochemistry |
| url | https://ir.uitm.edu.my/id/eprint/35569/1/35569.PDF |
| work_keys_str_mv | AT harunaiza influenceofwateractivityontheenantioselectiveesterificationcatalysedbyhydrogelimmobilisedlipasefromcandidarugosaaizaharun |