Thermotolerance of lactobacillus rhamnosus NRRL 442 encapsulated in bagasse-alginate microcapsule
Thesis (PhD. (Bioprocess Engineering))
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| Format: | Thesis |
| Language: | English |
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Universiti Teknologi Malaysia
2024
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| Online Access: | http://openscience.utm.my/handle/123456789/1216 |
| _version_ | 1825623280426418176 |
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| author | Shaharuddin, Shahrulzaman |
| author_facet | Shaharuddin, Shahrulzaman |
| author_sort | Shaharuddin, Shahrulzaman |
| collection | OpenScience |
| description | Thesis (PhD. (Bioprocess Engineering)) |
| first_indexed | 2024-09-23T23:50:10Z |
| format | Thesis |
| id | oai:openscience.utm.my:123456789/1216 |
| institution | Universiti Teknologi Malaysia - OpenScience |
| language | English |
| last_indexed | 2024-09-23T23:50:10Z |
| publishDate | 2024 |
| publisher | Universiti Teknologi Malaysia |
| record_format | dspace |
| spelling | oai:openscience.utm.my:123456789/12162024-06-22T18:00:26Z Thermotolerance of lactobacillus rhamnosus NRRL 442 encapsulated in bagasse-alginate microcapsule Shaharuddin, Shahrulzaman Lactobacillus Probiotics Pelletizing Thesis (PhD. (Bioprocess Engineering)) Normally, probiotic is applied in its initial form before mixed with other ingredients in pelleted animal feed. During heat-treatment in pelleting process, the viability and effective activity of the probiotic reduce significantly. This research aimed to design a thermotolerant microcapsule of immobilized probiotic Lactobacillus rhamnosus NRRL 442 using sugarcane bagasse-alginate composite as encapsulant agent. The microcapsule was produced by combination of immobilization and microencapsulation techniques via adsorption and extrusion, respectively. Pretreated sugarcane bagasses (SB) were used as probiotic carrier. Firstly, L.rhamnosus (Lr) was immobilized with the SB. Then the microcapsules of immobilized probiotic on bagasse-alginate (NaA-SB) composite were synthesized using extrusion method to provide double protection effect to the probiotic. It was found that high immobilization efficiency of L.rhamnosus (97.67% ± 0.42) can be obtained. From scanning eletron microscope (SEM) micrographs, the attachment of L.rhamnosus was observed mainly on the SB surface with almost similar structural properties as compared with the raw SB. Microencapsulation process with ratio of NaA:SB of 1:1.5 and 1:1 attained the highest efficiency (89.35% ± 0.49 and 89.35% ± 0.21, respectively). The highest cell survivability after the heat exposure was observed in the sample synthesized using 3% of NaA and NaA:SB ratio of 1:1.5 (81.30±0.30%). The recommended parameters for immobilization and microencapsulation process were 1:8 of the SB:L.rhamnosus ratio and 3% of NaA concentration with NaA:SB ratio of 1:1.5, respectively. The step of probiotic immobilization before microencapsulation significantly enhanced microencapsulation efficiency and cell survivability following heat exposure of 90 ºC for 30 seconds. SEM images after the heat exposure analysis showed the evidence of survived probiotic in bagasse-alginate microcapsule. The thermotolerance of probiotic increased with the increase of microcapsule diameter. In addition, fourier transform infrared spectroscopy spectra confirmed the improvement in functional bonding with the synergical presence of sugarcane bagasse, probiotic and alginate. Hence, the cell survivability after heat exposure was enhanced using double heat protection via microencapsulation of immobilized probiotic. Heat movement and the mechanism of heat resistance during the heat exposure were illustrated. The regression model exhibited strong correlation between effective thermal conductivity and encapsulant concentration whereby these results were in good agreement with cell survivability results. These findings demonstrated a great potential of heat resistant microcapsules for probiotic and then for inclusion as probiotic additive Faculty of Chemical Engineering 2024-06-22T09:53:42Z 2024-06-22T09:53:42Z 2015 Thesis Dataset http://openscience.utm.my/handle/123456789/1216 en application/pdf application/pdf application/pdf application/pdf Universiti Teknologi Malaysia |
| spellingShingle | Lactobacillus Probiotics Pelletizing Shaharuddin, Shahrulzaman Thermotolerance of lactobacillus rhamnosus NRRL 442 encapsulated in bagasse-alginate microcapsule |
| title | Thermotolerance of lactobacillus rhamnosus NRRL 442 encapsulated in bagasse-alginate microcapsule |
| title_full | Thermotolerance of lactobacillus rhamnosus NRRL 442 encapsulated in bagasse-alginate microcapsule |
| title_fullStr | Thermotolerance of lactobacillus rhamnosus NRRL 442 encapsulated in bagasse-alginate microcapsule |
| title_full_unstemmed | Thermotolerance of lactobacillus rhamnosus NRRL 442 encapsulated in bagasse-alginate microcapsule |
| title_short | Thermotolerance of lactobacillus rhamnosus NRRL 442 encapsulated in bagasse-alginate microcapsule |
| title_sort | thermotolerance of lactobacillus rhamnosus nrrl 442 encapsulated in bagasse alginate microcapsule |
| topic | Lactobacillus Probiotics Pelletizing |
| url | http://openscience.utm.my/handle/123456789/1216 |
| work_keys_str_mv | AT shaharuddinshahrulzaman thermotoleranceoflactobacillusrhamnosusnrrl442encapsulatedinbagassealginatemicrocapsule |