Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro
Giardia intestinalis (G.I), is an anaerobic protozoan and the aetiological agent of giardiasis, a diarrhoea present worldwide and associated with poverty. G.I has a simple life cycle alternating between cyst and trophozoite. Cysts are transmitted orally to the stomach and transform to trophozoites i...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | https://repository.londonmet.ac.uk/1204/1/1-s2.0-S0171933516302400-main.pdf |
| _version_ | 1825624749822181376 |
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| author | Evans-Osses, Ingrid Mojoli, Andres Monguió-Tortajada, Marta Marcilla, Antonio Aran, Veronica Amorim, Maria Inal, Jameel Borràs, Francesc E. Ramirez, Marcel I. |
| author_facet | Evans-Osses, Ingrid Mojoli, Andres Monguió-Tortajada, Marta Marcilla, Antonio Aran, Veronica Amorim, Maria Inal, Jameel Borràs, Francesc E. Ramirez, Marcel I. |
| author_sort | Evans-Osses, Ingrid |
| collection | LMU |
| description | Giardia intestinalis (G.I), is an anaerobic protozoan and the aetiological agent of giardiasis, a diarrhoea present worldwide and associated with poverty. G.I has a simple life cycle alternating between cyst and trophozoite. Cysts are transmitted orally to the stomach and transform to trophozoites in the intestine by a multifactorial process. Recently, microvesicles (MVs) have been found to be released from a wide range of eukaryotic cells. We have observed a release of MVs during the life cycle of G.I., identifying MVs from active trophozoites and from trophozoites differentiating to the cyst form. The aim of the current work was to investigate the role of MVs from G.I in the pathogenesis of giardiasis. MVs from log phase were able to increase the attachment of G. intestinalis trophozoites to Caco-2 cells. Moreover, MVs from G. intestinalis could be captured by human immature dendritic cells, resulting in increased activation and allostimulation of human dendritic cells. Lipid rafts participate in the MV biogenesis and in the attachment to Caco-2 cells. Nevertheless, proteomic analysis from two types of MVs has shown slight differences at the protein levels. An understanding of biogenesis and content of MVs derived from trophozoites might have important implications in the pathogenesis of the disease. |
| first_indexed | 2024-07-09T03:46:32Z |
| format | Article |
| id | oai:repository.londonmet.ac.uk:1204 |
| institution | London Metropolitan University |
| language | English |
| last_indexed | 2024-07-09T03:46:32Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | eprints |
| spelling | oai:repository.londonmet.ac.uk:12042020-05-29T16:31:40Z https://repository.londonmet.ac.uk/1204/ Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro Evans-Osses, Ingrid Mojoli, Andres Monguió-Tortajada, Marta Marcilla, Antonio Aran, Veronica Amorim, Maria Inal, Jameel Borràs, Francesc E. Ramirez, Marcel I. 570 Life sciences; biology 610 Medicine & health Giardia intestinalis (G.I), is an anaerobic protozoan and the aetiological agent of giardiasis, a diarrhoea present worldwide and associated with poverty. G.I has a simple life cycle alternating between cyst and trophozoite. Cysts are transmitted orally to the stomach and transform to trophozoites in the intestine by a multifactorial process. Recently, microvesicles (MVs) have been found to be released from a wide range of eukaryotic cells. We have observed a release of MVs during the life cycle of G.I., identifying MVs from active trophozoites and from trophozoites differentiating to the cyst form. The aim of the current work was to investigate the role of MVs from G.I in the pathogenesis of giardiasis. MVs from log phase were able to increase the attachment of G. intestinalis trophozoites to Caco-2 cells. Moreover, MVs from G. intestinalis could be captured by human immature dendritic cells, resulting in increased activation and allostimulation of human dendritic cells. Lipid rafts participate in the MV biogenesis and in the attachment to Caco-2 cells. Nevertheless, proteomic analysis from two types of MVs has shown slight differences at the protein levels. An understanding of biogenesis and content of MVs derived from trophozoites might have important implications in the pathogenesis of the disease. Elsevier 2017-01-22 Article PeerReviewed text en cc_by_nc_nd https://repository.londonmet.ac.uk/1204/1/1-s2.0-S0171933516302400-main.pdf Evans-Osses, Ingrid, Mojoli, Andres, Monguió-Tortajada, Marta, Marcilla, Antonio, Aran, Veronica, Amorim, Maria, Inal, Jameel, Borràs, Francesc E. and Ramirez, Marcel I. (2017) Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro. European Journal of Cell Biology. ISSN 0171-9335 http://www.sciencedirect.com/science/article/pii/S0171933516302400 10.1016/j.ejcb.2017.01.005 10.1016/j.ejcb.2017.01.005 |
| spellingShingle | 570 Life sciences; biology 610 Medicine & health Evans-Osses, Ingrid Mojoli, Andres Monguió-Tortajada, Marta Marcilla, Antonio Aran, Veronica Amorim, Maria Inal, Jameel Borràs, Francesc E. Ramirez, Marcel I. Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro |
| title | Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro |
| title_full | Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro |
| title_fullStr | Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro |
| title_full_unstemmed | Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro |
| title_short | Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro |
| title_sort | microvesicles released from giardia intestinalis disturb host pathogen response in vitro |
| topic | 570 Life sciences; biology 610 Medicine & health |
| url | https://repository.londonmet.ac.uk/1204/1/1-s2.0-S0171933516302400-main.pdf |
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