Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets
This study sought to measure medium-sized extracellular vesicles (mEVs) in plasma, when patients have low Plasmodium falciparum early in infection. We aimed to define the relationship between plasma mEVs and: (i) parasitaemia, (ii) period from onset of malaria symptoms until seeking medical care (pa...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2019
|
| Subjects: | |
| Online Access: | https://repository.londonmet.ac.uk/5413/1/Plasma-mEV-levels-in-Ghanain-malaria-patients-with-low-parasitaemia-are-higher-than-those-of-healthy-controls-raising-the-potential-for-parasite.pdf |
| _version_ | 1825625431546527744 |
|---|---|
| author | Antwi-Baffour, Samuel Malibha-Pinchbeck, Memory Stratton, Dan Jorfi, Samireh Lange, Sigrun Inal, Jameel |
| author_facet | Antwi-Baffour, Samuel Malibha-Pinchbeck, Memory Stratton, Dan Jorfi, Samireh Lange, Sigrun Inal, Jameel |
| author_sort | Antwi-Baffour, Samuel |
| collection | LMU |
| description | This study sought to measure medium-sized extracellular vesicles (mEVs) in plasma, when patients have low Plasmodium falciparum early in infection. We aimed to define the relationship between plasma mEVs and: (i) parasitaemia, (ii) period from onset of malaria symptoms until seeking medical care (patient delay, PD), (iii) age and (iv) gender. In this cross-sectional study, n = 434 patients were analysed and Nanosight Tracking Analysis (NTA) used to quantify mEVs (vesicles of 150–500 nm diameter, isolated at 15,000 × g, β-tubulin-positive and staining for annexin V, but weak or negative for CD81). Overall plasma mEV levels (1.69 × 1010 mEVs mL−1) were 2.3-fold higher than for uninfected controls (0.51 × 1010 mEVs mL−1). Divided into four age groups, we found a bimodal distribution with 2.5- and 2.1-fold higher mEVs in infected children (<11 years old [yo]) (median:2.11 × 1010 mEVs mL−1) and the elderly (>45 yo) (median:1.92 × 1010 mEVs mL−1), respectively, compared to uninfected controls; parasite density varied similarly with age groups. There was a positive association between mEVs and parasite density (r = 0.587, p < 0.0001) and mEVs were strongly associated with PD (r = 0.919, p < 0.0001), but gender had no effect on plasma mEV levels (p = 0.667). Parasite density was also exponentially related to patient delay. Gender (p = 0.667) had no effect on plasma mEV levels. During periods of low parasitaemia (PD = 72h), mEVs were 0.93-fold greater than in uninfected controls. As 75% (49/65) of patients had low parasitaemia levels (20–500 parasites µL−1), close to the detection limits of microscopy of Giemsa-stained thick blood films (5–150 parasites µL−1), mEV quantification by NTA could potentially have early diagnostic value, and raises the potential of Pf markers in mEVs as early diagnostic targets. |
| first_indexed | 2024-07-09T03:59:32Z |
| format | Article |
| id | oai:repository.londonmet.ac.uk:5413 |
| institution | London Metropolitan University |
| language | English |
| last_indexed | 2024-07-09T03:59:32Z |
| publishDate | 2019 |
| publisher | Taylor & Francis |
| record_format | eprints |
| spelling | oai:repository.londonmet.ac.uk:54132020-01-21T09:31:48Z https://repository.londonmet.ac.uk/5413/ Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets Antwi-Baffour, Samuel Malibha-Pinchbeck, Memory Stratton, Dan Jorfi, Samireh Lange, Sigrun Inal, Jameel 570 Life sciences; biology 610 Medicine & health This study sought to measure medium-sized extracellular vesicles (mEVs) in plasma, when patients have low Plasmodium falciparum early in infection. We aimed to define the relationship between plasma mEVs and: (i) parasitaemia, (ii) period from onset of malaria symptoms until seeking medical care (patient delay, PD), (iii) age and (iv) gender. In this cross-sectional study, n = 434 patients were analysed and Nanosight Tracking Analysis (NTA) used to quantify mEVs (vesicles of 150–500 nm diameter, isolated at 15,000 × g, β-tubulin-positive and staining for annexin V, but weak or negative for CD81). Overall plasma mEV levels (1.69 × 1010 mEVs mL−1) were 2.3-fold higher than for uninfected controls (0.51 × 1010 mEVs mL−1). Divided into four age groups, we found a bimodal distribution with 2.5- and 2.1-fold higher mEVs in infected children (<11 years old [yo]) (median:2.11 × 1010 mEVs mL−1) and the elderly (>45 yo) (median:1.92 × 1010 mEVs mL−1), respectively, compared to uninfected controls; parasite density varied similarly with age groups. There was a positive association between mEVs and parasite density (r = 0.587, p < 0.0001) and mEVs were strongly associated with PD (r = 0.919, p < 0.0001), but gender had no effect on plasma mEV levels (p = 0.667). Parasite density was also exponentially related to patient delay. Gender (p = 0.667) had no effect on plasma mEV levels. During periods of low parasitaemia (PD = 72h), mEVs were 0.93-fold greater than in uninfected controls. As 75% (49/65) of patients had low parasitaemia levels (20–500 parasites µL−1), close to the detection limits of microscopy of Giemsa-stained thick blood films (5–150 parasites µL−1), mEV quantification by NTA could potentially have early diagnostic value, and raises the potential of Pf markers in mEVs as early diagnostic targets. Taylor & Francis 2019-12-18 Article PeerReviewed text en cc_by_nc_4 https://repository.londonmet.ac.uk/5413/1/Plasma-mEV-levels-in-Ghanain-malaria-patients-with-low-parasitaemia-are-higher-than-those-of-healthy-controls-raising-the-potential-for-parasite.pdf Antwi-Baffour, Samuel, Malibha-Pinchbeck, Memory, Stratton, Dan, Jorfi, Samireh, Lange, Sigrun and Inal, Jameel (2019) Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets. Journal of Extracellular Vesicles, 9 (1). pp. 1-13. ISSN 2001-3078 10.1080/20013078.2019.1697124 10.1080/20013078.2019.1697124 |
| spellingShingle | 570 Life sciences; biology 610 Medicine & health Antwi-Baffour, Samuel Malibha-Pinchbeck, Memory Stratton, Dan Jorfi, Samireh Lange, Sigrun Inal, Jameel Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets |
| title | Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets |
| title_full | Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets |
| title_fullStr | Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets |
| title_full_unstemmed | Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets |
| title_short | Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets |
| title_sort | plasma mev levels in ghanain malaria patients with low parasitaemia are higher than those of healthy controls raising the potential for parasite markers in mevs as diagnostic targets |
| topic | 570 Life sciences; biology 610 Medicine & health |
| url | https://repository.londonmet.ac.uk/5413/1/Plasma-mEV-levels-in-Ghanain-malaria-patients-with-low-parasitaemia-are-higher-than-those-of-healthy-controls-raising-the-potential-for-parasite.pdf |
| work_keys_str_mv | AT antwibaffoursamuel plasmamevlevelsinghanainmalariapatientswithlowparasitaemiaarehigherthanthoseofhealthycontrolsraisingthepotentialforparasitemarkersinmevsasdiagnostictargets AT malibhapinchbeckmemory plasmamevlevelsinghanainmalariapatientswithlowparasitaemiaarehigherthanthoseofhealthycontrolsraisingthepotentialforparasitemarkersinmevsasdiagnostictargets AT strattondan plasmamevlevelsinghanainmalariapatientswithlowparasitaemiaarehigherthanthoseofhealthycontrolsraisingthepotentialforparasitemarkersinmevsasdiagnostictargets AT jorfisamireh plasmamevlevelsinghanainmalariapatientswithlowparasitaemiaarehigherthanthoseofhealthycontrolsraisingthepotentialforparasitemarkersinmevsasdiagnostictargets AT langesigrun plasmamevlevelsinghanainmalariapatientswithlowparasitaemiaarehigherthanthoseofhealthycontrolsraisingthepotentialforparasitemarkersinmevsasdiagnostictargets AT inaljameel plasmamevlevelsinghanainmalariapatientswithlowparasitaemiaarehigherthanthoseofhealthycontrolsraisingthepotentialforparasitemarkersinmevsasdiagnostictargets |