Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022
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| Online Access: | https://repository.londonmet.ac.uk/7852/1/WIREs-Nanomed-Nanobiotechnol-2022-Isolation-and-characterization-of-extracellular-vesicles-and-future.pdf |
| _version_ | 1804072829282418688 |
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| author | De Sousa, Karina P. Rossi, Izadora Abdullahi, Mahamed Ramirez, Marcel Ivan Stratton, Dan Inal, Jameel |
| author_facet | De Sousa, Karina P. Rossi, Izadora Abdullahi, Mahamed Ramirez, Marcel Ivan Stratton, Dan Inal, Jameel |
| author_sort | De Sousa, Karina P. |
| collection | LMU |
| description | Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV-related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. |
| first_indexed | 2024-07-09T04:05:22Z |
| format | Article |
| id | oai:repository.londonmet.ac.uk:7852 |
| institution | London Metropolitan University |
| language | English |
| last_indexed | 2024-07-09T04:05:22Z |
| publishDate | 2022 |
| publisher | Wiley |
| record_format | eprints |
| spelling | oai:repository.londonmet.ac.uk:78522023-02-20T12:09:32Z http://repository.londonmet.ac.uk/7852/ Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy De Sousa, Karina P. Rossi, Izadora Abdullahi, Mahamed Ramirez, Marcel Ivan Stratton, Dan Inal, Jameel 610 Medicine & health Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV-related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. Wiley 2022-07-27 Article PeerReviewed text en cc_by_4 https://repository.londonmet.ac.uk/7852/1/WIREs-Nanomed-Nanobiotechnol-2022-Isolation-and-characterization-of-extracellular-vesicles-and-future.pdf De Sousa, Karina P., Rossi, Izadora, Abdullahi, Mahamed, Ramirez, Marcel Ivan, Stratton, Dan and Inal, Jameel (2022) Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology, 15(1) (e1835). pp. 1-29. ISSN 1939-0041 https://doi.org/10.1002/wnan.1835 10.1002/wnan.1835 |
| spellingShingle | 610 Medicine & health De Sousa, Karina P. Rossi, Izadora Abdullahi, Mahamed Ramirez, Marcel Ivan Stratton, Dan Inal, Jameel Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
| title | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
| title_full | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
| title_fullStr | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
| title_full_unstemmed | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
| title_short | Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
| title_sort | isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy |
| topic | 610 Medicine & health |
| url | https://repository.londonmet.ac.uk/7852/1/WIREs-Nanomed-Nanobiotechnol-2022-Isolation-and-characterization-of-extracellular-vesicles-and-future.pdf |
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