Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product
Background: Antimicrobial resistance (AMR) is one of the biggest threats to public health. The food chain has been recognised as a vehicle for transmitting AMR bacteria. However, information about resistant strains isolated from African traditional fermented foods remains limited. Nono is a tradit...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023
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| Online Access: | https://repository.londonmet.ac.uk/8535/1/antibiotics-12-00843.pdf |
| _version_ | 1804072917057667072 |
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| author | Obioha, Promiselynda Ijeoma Anyogu, Amarachukwu Awamaria, Brigitte Ghoddusi, Hamid Ouoba, Irene |
| author_facet | Obioha, Promiselynda Ijeoma Anyogu, Amarachukwu Awamaria, Brigitte Ghoddusi, Hamid Ouoba, Irene |
| author_sort | Obioha, Promiselynda Ijeoma |
| collection | LMU |
| description | Background:
Antimicrobial resistance (AMR) is one of the biggest threats to public health. The food chain has been recognised as a vehicle for transmitting AMR bacteria. However, information about resistant strains isolated from African traditional fermented foods remains limited. Nono is a traditional, naturally fermented milk product consumed by many pastoral communities across West Africa. The main aim of this study was to investigate and determine the AMR patterns of lactic acid bacteria (LAB) involved in the traditional fermentation of milk for Nono production, and the presence of transferable AMR determinants.
Methods:
One hundred (100) LAB isolates from Nono identified in a previous study as Limosilactobacillus fermentum, Lactobacillus delbrueckii, Streptococcus thermophilus, Streptococcus infantarius, Lentilactobacillus senioris, Leuconostoc pseudomesenteriodes, and Enterococcus thailandicus were investigated. The minimum inhibitory concentration (MIC) was determined for 18 antimicrobials using the micro-broth dilution method. In addition, LAB isolates were screened for 28 antimicrobial resistance genes using PCR. The ability of LAB isolates to transfer tetracycline and streptomycin resistance genes to Enterococcus faecalis was also investigated.
Results:
The experiments revealed variable antimicrobial susceptibility according to the LAB isolate and the antimicrobial tested. The tetracycline resistance genes tet(S) and tet(M) were detected in isolates Ent. thailandicus 52 and S. infantarius 10. Additionally, aad(E) encoding resistance to streptomycin was detected in Ent. thailandicus 52. The conjugation experiments suggested that the tet(S) and aad(E) genes were transferable in vitro from isolate Ent. thailandicus 52 to Ent. faecalis JH2-2.
Significance and Impact:
Traditional fermented foods play a significant role in the diet of millions of people in Africa, yet their contribution to the burden of AMR is largely unknown. This study highlights that LAB involved in traditionally fermented foods could be potential reservoirs of AMR. It also underscores the relevant safety issues of Ent. thailandicus 52 and S. infantarius 10 for use as starter cultures as they carry transferable AMR genes. Starter cultures are an essential aspect of improving the safety and quality attributes of African fermented foods. However, AMR monitoring is an important safety aspect in the selection of starter cultures for improving traditional fermentation technologies. |
| first_indexed | 2024-07-09T04:06:45Z |
| format | Article |
| id | oai:repository.londonmet.ac.uk:8535 |
| institution | London Metropolitan University |
| language | English |
| last_indexed | 2024-07-09T04:06:45Z |
| publishDate | 2023 |
| publisher | MDPI AG |
| record_format | eprints |
| spelling | oai:repository.londonmet.ac.uk:85352023-05-23T11:52:26Z http://repository.londonmet.ac.uk/8535/ Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product Obioha, Promiselynda Ijeoma Anyogu, Amarachukwu Awamaria, Brigitte Ghoddusi, Hamid Ouoba, Irene 570 Life sciences; biology 610 Medicine & health Background: Antimicrobial resistance (AMR) is one of the biggest threats to public health. The food chain has been recognised as a vehicle for transmitting AMR bacteria. However, information about resistant strains isolated from African traditional fermented foods remains limited. Nono is a traditional, naturally fermented milk product consumed by many pastoral communities across West Africa. The main aim of this study was to investigate and determine the AMR patterns of lactic acid bacteria (LAB) involved in the traditional fermentation of milk for Nono production, and the presence of transferable AMR determinants. Methods: One hundred (100) LAB isolates from Nono identified in a previous study as Limosilactobacillus fermentum, Lactobacillus delbrueckii, Streptococcus thermophilus, Streptococcus infantarius, Lentilactobacillus senioris, Leuconostoc pseudomesenteriodes, and Enterococcus thailandicus were investigated. The minimum inhibitory concentration (MIC) was determined for 18 antimicrobials using the micro-broth dilution method. In addition, LAB isolates were screened for 28 antimicrobial resistance genes using PCR. The ability of LAB isolates to transfer tetracycline and streptomycin resistance genes to Enterococcus faecalis was also investigated. Results: The experiments revealed variable antimicrobial susceptibility according to the LAB isolate and the antimicrobial tested. The tetracycline resistance genes tet(S) and tet(M) were detected in isolates Ent. thailandicus 52 and S. infantarius 10. Additionally, aad(E) encoding resistance to streptomycin was detected in Ent. thailandicus 52. The conjugation experiments suggested that the tet(S) and aad(E) genes were transferable in vitro from isolate Ent. thailandicus 52 to Ent. faecalis JH2-2. Significance and Impact: Traditional fermented foods play a significant role in the diet of millions of people in Africa, yet their contribution to the burden of AMR is largely unknown. This study highlights that LAB involved in traditionally fermented foods could be potential reservoirs of AMR. It also underscores the relevant safety issues of Ent. thailandicus 52 and S. infantarius 10 for use as starter cultures as they carry transferable AMR genes. Starter cultures are an essential aspect of improving the safety and quality attributes of African fermented foods. However, AMR monitoring is an important safety aspect in the selection of starter cultures for improving traditional fermentation technologies. MDPI AG 2023-05-02 Article PeerReviewed text en cc_by_4 https://repository.londonmet.ac.uk/8535/1/antibiotics-12-00843.pdf Obioha, Promiselynda Ijeoma, Anyogu, Amarachukwu, Awamaria, Brigitte, Ghoddusi, Hamid and Ouoba, Irene (2023) Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product. Antibiotics, 12 (5) (843). pp. 1-14. ISSN 2079-6382 https://doi.org/10.3390/antibiotics12050843 10.3390/antibiotics12050843 |
| spellingShingle | 570 Life sciences; biology 610 Medicine & health Obioha, Promiselynda Ijeoma Anyogu, Amarachukwu Awamaria, Brigitte Ghoddusi, Hamid Ouoba, Irene Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product |
| title | Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product |
| title_full | Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product |
| title_fullStr | Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product |
| title_full_unstemmed | Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product |
| title_short | Antimicrobial resistance of lactic acid bacteria from Nono, a naturally fermented milk product |
| title_sort | antimicrobial resistance of lactic acid bacteria from nono a naturally fermented milk product |
| topic | 570 Life sciences; biology 610 Medicine & health |
| url | https://repository.londonmet.ac.uk/8535/1/antibiotics-12-00843.pdf |
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