The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo.
Fractalkine (CX3CL1) is synthesized as a type I transmembrane protein. Its unique CX(3)C chemokine domain is attached to a 241-amino acid mucin stalk, a 19-amino acid transmembrane domain, and a 37-amino acid intracellular domain of unknown function. A soluble form of fractalkine can be generated by...
| Үндсэн зохиолчид: | , , , , , , |
|---|---|
| Формат: | Journal article |
| Хэл сонгох: | English |
| Хэвлэсэн: |
2001
|
| _version_ | 1826256477580427264 |
|---|---|
| author | Lucas, A Chadwick, N Warren, B Jewell, D Gordon, S Powrie, F Greaves, D |
| author_facet | Lucas, A Chadwick, N Warren, B Jewell, D Gordon, S Powrie, F Greaves, D |
| author_sort | Lucas, A |
| collection | OXFORD |
| description | Fractalkine (CX3CL1) is synthesized as a type I transmembrane protein. Its unique CX(3)C chemokine domain is attached to a 241-amino acid mucin stalk, a 19-amino acid transmembrane domain, and a 37-amino acid intracellular domain of unknown function. A soluble form of fractalkine can be generated by proteolytic cleavage at the base of the mucin stalk. Novel monoclonal and polyclonal antibodies that specifically recognize only the amino- or carboxyl-terminal ends of the human fractalkine molecule have revealed that epithelial cells are the predominant cell type expressing transmembrane forms of fractalkine in human skin, the tonsil, and the large intestine. Using these specific anti-fractalkine reagents we do not detect high-level expression of fractalkine on endothelial cells in normal or inflamed colon samples obtained from patients with Crohn's disease or ulcerative colitis. In contrast to previous reports we do not detect fractalkine expression by Langerhans cells or immature dendritic cells in mucosal-associated lymphoid tissues in vivo. We show that the reagent used in previous studies, an anti-fractalkine N-terminal peptide antisera, cross-reacts with human CD84. Finally we discuss potential roles for fractalkine in constitutive leukocyte trafficking based on its observed pattern of expression in epithelia. |
| first_indexed | 2024-03-06T18:02:51Z |
| format | Journal article |
| id | oxford-uuid:00688d02-69f7-43f7-9a98-cc2c9bf67566 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:02:51Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:00688d02-69f7-43f7-9a98-cc2c9bf675662022-03-26T08:29:26ZThe transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:00688d02-69f7-43f7-9a98-cc2c9bf67566EnglishSymplectic Elements at Oxford2001Lucas, AChadwick, NWarren, BJewell, DGordon, SPowrie, FGreaves, DFractalkine (CX3CL1) is synthesized as a type I transmembrane protein. Its unique CX(3)C chemokine domain is attached to a 241-amino acid mucin stalk, a 19-amino acid transmembrane domain, and a 37-amino acid intracellular domain of unknown function. A soluble form of fractalkine can be generated by proteolytic cleavage at the base of the mucin stalk. Novel monoclonal and polyclonal antibodies that specifically recognize only the amino- or carboxyl-terminal ends of the human fractalkine molecule have revealed that epithelial cells are the predominant cell type expressing transmembrane forms of fractalkine in human skin, the tonsil, and the large intestine. Using these specific anti-fractalkine reagents we do not detect high-level expression of fractalkine on endothelial cells in normal or inflamed colon samples obtained from patients with Crohn's disease or ulcerative colitis. In contrast to previous reports we do not detect fractalkine expression by Langerhans cells or immature dendritic cells in mucosal-associated lymphoid tissues in vivo. We show that the reagent used in previous studies, an anti-fractalkine N-terminal peptide antisera, cross-reacts with human CD84. Finally we discuss potential roles for fractalkine in constitutive leukocyte trafficking based on its observed pattern of expression in epithelia. |
| spellingShingle | Lucas, A Chadwick, N Warren, B Jewell, D Gordon, S Powrie, F Greaves, D The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. |
| title | The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. |
| title_full | The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. |
| title_fullStr | The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. |
| title_full_unstemmed | The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. |
| title_short | The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. |
| title_sort | transmembrane form of the cx3cl1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo |
| work_keys_str_mv | AT lucasa thetransmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT chadwickn thetransmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT warrenb thetransmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT jewelld thetransmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT gordons thetransmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT powrief thetransmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT greavesd thetransmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT lucasa transmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT chadwickn transmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT warrenb transmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT jewelld transmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT gordons transmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT powrief transmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo AT greavesd transmembraneformofthecx3cl1chemokinefractalkineisexpressedpredominantlybyepithelialcellsinvivo |