Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand.
There is a lack of diagnostic tests for leptospirosis in technology-restricted settings. We developed loop-mediated isothermal amplification (LAMP) specific for the 16S ribosomal RNA gene (rrs) of pathogenic and intermediate group Leptospira species. The lower limit of detection was 10 genomic equiv...
| Asıl Yazarlar: | , , , , , , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
2011
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| _version_ | 1826256562924027904 |
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| author | Sonthayanon, P Chierakul, W Wuthiekanun, V Thaipadungpanit, J Kalambaheti, T Boonsilp, S Amornchai, P Smythe, L Limmathurotsakul, D Day, N Peacock, S |
| author_facet | Sonthayanon, P Chierakul, W Wuthiekanun, V Thaipadungpanit, J Kalambaheti, T Boonsilp, S Amornchai, P Smythe, L Limmathurotsakul, D Day, N Peacock, S |
| author_sort | Sonthayanon, P |
| collection | OXFORD |
| description | There is a lack of diagnostic tests for leptospirosis in technology-restricted settings. We developed loop-mediated isothermal amplification (LAMP) specific for the 16S ribosomal RNA gene (rrs) of pathogenic and intermediate group Leptospira species. The lower limit of detection was 10 genomic equivalents/reaction, and analytical specificity was high; we observed positive reactions for pathogenic/intermediate groups and negative reactions for non-pathogenic Leptospira species and other bacterial species. We evaluated this assay in Thailand by using a case-control study of 133 patients with laboratory-proven leptospirosis and 133 patients with other febrile illnesses. Using admission blood, we found that the rrs LAMP showed positive results in 58 of 133 cases (diagnostic sensitivity = 43.6, 95% confidence interval [CI] = 35.0-52.5) and in 22 of 133 controls (diagnostic specificity = 83.5, 95% CI = 76.0-89.3). Sensitivity was high for 39 patients who were culture positive for Leptospira spp. (84.6, 95% CI = 69.5-94.1). The rrs LAMP can provide an admission diagnosis in approximately half of patients with leptospirosis, but its clinical utility is reduced by a lower specificity. |
| first_indexed | 2024-03-06T18:04:12Z |
| format | Journal article |
| id | oxford-uuid:00df7da9-abdd-48ec-bd06-0587c55328a8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:04:12Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:00df7da9-abdd-48ec-bd06-0587c55328a82022-03-26T08:31:52ZAccuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:00df7da9-abdd-48ec-bd06-0587c55328a8EnglishSymplectic Elements at Oxford2011Sonthayanon, PChierakul, WWuthiekanun, VThaipadungpanit, JKalambaheti, TBoonsilp, SAmornchai, PSmythe, LLimmathurotsakul, DDay, NPeacock, SThere is a lack of diagnostic tests for leptospirosis in technology-restricted settings. We developed loop-mediated isothermal amplification (LAMP) specific for the 16S ribosomal RNA gene (rrs) of pathogenic and intermediate group Leptospira species. The lower limit of detection was 10 genomic equivalents/reaction, and analytical specificity was high; we observed positive reactions for pathogenic/intermediate groups and negative reactions for non-pathogenic Leptospira species and other bacterial species. We evaluated this assay in Thailand by using a case-control study of 133 patients with laboratory-proven leptospirosis and 133 patients with other febrile illnesses. Using admission blood, we found that the rrs LAMP showed positive results in 58 of 133 cases (diagnostic sensitivity = 43.6, 95% confidence interval [CI] = 35.0-52.5) and in 22 of 133 controls (diagnostic specificity = 83.5, 95% CI = 76.0-89.3). Sensitivity was high for 39 patients who were culture positive for Leptospira spp. (84.6, 95% CI = 69.5-94.1). The rrs LAMP can provide an admission diagnosis in approximately half of patients with leptospirosis, but its clinical utility is reduced by a lower specificity. |
| spellingShingle | Sonthayanon, P Chierakul, W Wuthiekanun, V Thaipadungpanit, J Kalambaheti, T Boonsilp, S Amornchai, P Smythe, L Limmathurotsakul, D Day, N Peacock, S Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand. |
| title | Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand. |
| title_full | Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand. |
| title_fullStr | Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand. |
| title_full_unstemmed | Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand. |
| title_short | Accuracy of loop-mediated isothermal amplification for diagnosis of human leptospirosis in Thailand. |
| title_sort | accuracy of loop mediated isothermal amplification for diagnosis of human leptospirosis in thailand |
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