Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families.
BACKGROUND: Respiratory syncytial virus (RSV) infects nearly all children by the end of their second winter. Why some develop bronchiolitis is poorly understood; it is not known whether there is a genetic component. The pathological features include neutrophil infiltration and high levels of interle...
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| Format: | Journal article |
| Language: | English |
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2000
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| _version_ | 1797050387844300800 |
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| author | Hull, J Thomson, A Kwiatkowski, D |
| author_facet | Hull, J Thomson, A Kwiatkowski, D |
| author_sort | Hull, J |
| collection | OXFORD |
| description | BACKGROUND: Respiratory syncytial virus (RSV) infects nearly all children by the end of their second winter. Why some develop bronchiolitis is poorly understood; it is not known whether there is a genetic component. The pathological features include neutrophil infiltration and high levels of interleukin 8 (IL-8), a potent neutrophil chemoattractant. METHODS: Common genetic variants of the promoter region of the IL-8 gene were identified by sequencing DNA from 36 healthy individuals. Genetic correlates of IL-8 production were assessed using whole blood from 50 healthy subjects. To investigate genetic correlates of disease severity 117 nuclear families were recruited in which a child had required hospital admission for RSV bronchiolitis. RESULTS: A common single nucleotide polymorphism (allele frequency 0.44) was identified 251 bp upstream of the IL-8 transcription start site. The IL8-251A allele tended to be associated with increased IL-8 production by lipopolysaccharide stimulated whole blood (p=0.07). Using the transmission disequilibrium test, the frequency of this allele was significantly increased in infants with bronchiolitis (transmission = 62% (95% confidence interval (CI) 53 to 71), p=0.014) and particularly in those without known risk factors (transmission = 78% (95% CI 62 to 93), p=0.004). CONCLUSION: Disease severity following RSV infection appears to be determined by a genetic factor close to the IL-8 gene. Further analysis of this effect may elucidate causal processes in the pathogenesis of RSV bronchiolitis. |
| first_indexed | 2024-03-06T18:04:24Z |
| format | Journal article |
| id | oxford-uuid:00f0c03e-0876-4902-bc1c-a7a5509fba4b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:04:24Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:00f0c03e-0876-4902-bc1c-a7a5509fba4b2022-03-26T08:32:09ZAssociation of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:00f0c03e-0876-4902-bc1c-a7a5509fba4bEnglishSymplectic Elements at Oxford2000Hull, JThomson, AKwiatkowski, DBACKGROUND: Respiratory syncytial virus (RSV) infects nearly all children by the end of their second winter. Why some develop bronchiolitis is poorly understood; it is not known whether there is a genetic component. The pathological features include neutrophil infiltration and high levels of interleukin 8 (IL-8), a potent neutrophil chemoattractant. METHODS: Common genetic variants of the promoter region of the IL-8 gene were identified by sequencing DNA from 36 healthy individuals. Genetic correlates of IL-8 production were assessed using whole blood from 50 healthy subjects. To investigate genetic correlates of disease severity 117 nuclear families were recruited in which a child had required hospital admission for RSV bronchiolitis. RESULTS: A common single nucleotide polymorphism (allele frequency 0.44) was identified 251 bp upstream of the IL-8 transcription start site. The IL8-251A allele tended to be associated with increased IL-8 production by lipopolysaccharide stimulated whole blood (p=0.07). Using the transmission disequilibrium test, the frequency of this allele was significantly increased in infants with bronchiolitis (transmission = 62% (95% confidence interval (CI) 53 to 71), p=0.014) and particularly in those without known risk factors (transmission = 78% (95% CI 62 to 93), p=0.004). CONCLUSION: Disease severity following RSV infection appears to be determined by a genetic factor close to the IL-8 gene. Further analysis of this effect may elucidate causal processes in the pathogenesis of RSV bronchiolitis. |
| spellingShingle | Hull, J Thomson, A Kwiatkowski, D Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. |
| title | Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. |
| title_full | Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. |
| title_fullStr | Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. |
| title_full_unstemmed | Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. |
| title_short | Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. |
| title_sort | association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in uk families |
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