Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung.

Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung.

BACKGROUND: Existing methods of non-viral airway gene transfer suffer from low levels of efficiency. Electroporation has been used to enhance gene transfer in a range of tissues. Here we assess the usefulness of electroporation for enhancing gene transfer in the lungs of mice and sheep. METHODS: Na...

Full description

Bibliographic Details
Main Authors:	Pringle, I, McLachlan, G, Collie, D, Sumner-Jones, S, Lawton, A, Tennant, P, Baker, A, Gordon, C, Blundell, R, Varathalingam, A, Davies, L, Schmid, R, Cheng, S, Porteous, D, Gill, DR, Hyde, S
Format:	Journal article
Language:	English
Published:	2007

Similar Items

Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid-DNA complexes to ovine lung segments.
by: Emerson, M, et al.
Published: (2003)

CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
by: Hyde, S, et al.
Published: (2008)

Enhanced lung gene expression after aerosol delivery of concentrated pDNA/PEI complexes.
by: Davies, L, et al.
Published: (2008)

DEVELOPMENT, PRODUCTION AND EVALUATION OF CLINICAL GRADE CFTR EXPRESSION PLASMID FOR CF LUNG GENE THERAPY
by: Gill, DR, et al.
Published: (2010)

CpG-free plasmid expression cassettes for cystic fibrosis gene therapy
by: Pringle, I, et al.
Published: (2012)

CpG-free plasmid expression cassettes for cystic fibrosis gene therapy.
by: Pringle, I, et al.
Published: (2012)

3-STEP TAQMAN RT-PCR: ULTIMATE MESSENGER RNA DETECTION SENSITIVITY FOR CF GENE THERAPY CLINICAL TRIALS
by: McCormick, D, et al.
Published: (2009)

Biological efficacy and toxicity of naked plasmid DNA and cationic liposome-DNA complexes in ovine lung
by: Renwick, L, et al.
Published: (2003)

Optimizing aerosol gene delivery and expression in the ovine lung.
by: McLachlan, G, et al.
Published: (2007)

Long-term persistence of gene expression from adeno-associated virus serotype 5 in the mouse airways.
by: Sumner-Jones, S, et al.
Published: (2006)

Topical delivery of NF-kb decoy related oligodeoxynucleotides reduces pro-inflammatory cytokine response associated with cationic lipid: pDNA vector delivery to murine lungs
by: Varathalingam, et al.
Published: (2004)

Influence of CpG-dinucleotide motifs on the duration of gene expression from plasmid, vectors after in vivo lung delivery
by: Gill, DR, et al.
Published: (2007)

OPTIMISATION OF MOLECULAR ASSAYS FOR CLINICAL TRIAL OF GL67A/PDNA DELIVERY TO NOSE AND LUNG OF CF PATIENTS
by: Sumner-Jones, S, et al.
Published: (2009)

Transgene sequences free of CG dinucleotides lead to high level, long-term expression in the lung independent of plasmid backbone design
by: Bazzani, R, et al.
Published: (2016)

The use of CpG-free plasmids to mediate persistent gene expression following repeated aerosol delivery of pDNA/PEI complexes.
by: Davies, L, et al.
Published: (2012)

Progress and prospects: the design and production of plasmid vectors.
by: Gill, DR, et al.
Published: (2009)

The safety profile of a cationic lipid-mediated cystic fibrosis gene transfer agent following repeated monthly aerosol administration to sheep
by: Alton, E, et al.
Published: (2013)

The safety profile of a cationic lipid-mediated cystic fibrosis gene transfer agent following repeated monthly aerosol administration to sheep.
by: Alton, E, et al.
Published: (2013)

Electroporation-mediated interleukin-10 overexpression in skeletal muscle reduces acute rejection in rat cardiac allografts.
by: Tavakoli, R, et al.
Published: (2006)

Development of highly sensitive TaqMan RT-PCR assays for quantifying vector mRNA in human samples
by: Sumner-Jones, S, et al.
Published: (2008)

Intradermal electroporation of naked replicon RNA elicits strong immune responses.
by: Daniel X Johansson, et al.
Published: (2012-01-01)

REPEAT ADMINISTRATION OF GL67A/PGM169 IS FEASIBLE, SAFE, AND PRODUCES ENDOGENOUS LEVELS OF CFTR EXPRESSION AFTER 12 DOSES
by: Alton, E, et al.
Published: (2012)

CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
by: Hyde, Stephen C., et al.
Published: (2008)

Longevity of expression and levels of expression of gene therapy vectors using ovine air-liquid interface tracheal cells
by: Davidson, H, et al.
Published: (2007)

Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung.
by: Alton, E, et al.
Published: (2014)

Toxicology study assessing efficacy and safety of repeated administration of lipid/DNA complexes to mouse lung
by: Alton, E, et al.
Published: (2014)

In Utero Cortical Electroporation of Plasmids in the Mouse Embryo
by: Géraldine Meyer-Dilhet, et al.
Published: (2020-06-01)

Cumulative CFTR expression following repeated aerosol delivery of non-viral pGM169/GL67A formulation to mouse lung
by: Alton, E, et al.
Published: (2012)

Plasmid Inhalation: Delivery to the Airways
by: Davies, L, et al.
Published: (2006)

A novel large animal model for aerosol delivery of gene therapy vectors
by: McLachlan, G, et al.
Published: (2003)

Secreted Gaussia luciferase as a sensitive reporter gene for in vivo and ex vivo studies of airway gene transfer.
by: Griesenbach, U, et al.
Published: (2011)

743. Minicircles are similar to plasmids in providing high level, long-term expression in the lung
by: Gill, D, et al.
Published: (2016)

Injection and electroporation of plasmid DNA into human cortical organoids
by: Annina Denoth-Lippuner, et al.
Published: (2022-03-01)

Detection of plasmid DNA vectors following gene transfer to the murine airways.
by: Pringle, I, et al.
Published: (2005)

Adenovirus-mediated in utero expression of CFTR does not improve survival of CFTR knockout mice.
by: Davies, L, et al.
Published: (2008)

TOXICOLOGY STUDIES IN SUPPORT OF THE UK CF GENE THERAPY CONSORTIUM'S MULTI-DOSE CLINICAL TRIAL
by: Griesenbach, U, et al.
Published: (2011)

Development of an immunocytochemical assay to detect human CFTR expression following gene therapy
by: Davidson, H, et al.
Published: (2008)

Quantifying non-viral gene transfer by real-time PCR: a cautionary tale
by: Coles, R, et al.
Published: (2009)

Identification of transfected cell types following non-viral gene transfer to the murine lung.
by: Davies, L, et al.
Published: (2007)

Electrohydrodynamic comminution: a novel technique for the aerosolisation of plasmid DNA.
by: Davies, L, et al.
Published: (2005)