Chemical modification and micropatterning of Si(100) with oligonucleotides
A method for the reliable attachment of oligonucleotides to silicon (1 0 0) surfaces in patterns is described. UV light exposure of silicon (1 0 0) coated with alkenes functionalized with carboxylic acid groups and derivatives results in covalent attachment to the silicon surface. The carboxyl-termi...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1826256636833955840 |
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| author | Yin, H Brown, T Greef, R Wilkinson, J Melvin, T |
| author_facet | Yin, H Brown, T Greef, R Wilkinson, J Melvin, T |
| author_sort | Yin, H |
| collection | OXFORD |
| description | A method for the reliable attachment of oligonucleotides to silicon (1 0 0) surfaces in patterns is described. UV light exposure of silicon (1 0 0) coated with alkenes functionalized with carboxylic acid groups and derivatives results in covalent attachment to the silicon surface. The carboxyl-terminated (and derivatives) surfaces act as a substrate for the chemistry of the subsequent attachment of oligonucleotides. Illustrated are our results gained for optimisation of the surface attachment chemistry and the characterisation of the surface with scanning electron microscopy, epifluorescence microscopy and ellipsometry. © 2004 Elsevier B.V. All rights reserved. |
| first_indexed | 2024-03-06T18:05:23Z |
| format | Journal article |
| id | oxford-uuid:01424077-0927-4efe-b6d1-cd1d36b1fc2a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:05:23Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:01424077-0927-4efe-b6d1-cd1d36b1fc2a2022-03-26T08:33:56ZChemical modification and micropatterning of Si(100) with oligonucleotidesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:01424077-0927-4efe-b6d1-cd1d36b1fc2aEnglishSymplectic Elements at Oxford2004Yin, HBrown, TGreef, RWilkinson, JMelvin, TA method for the reliable attachment of oligonucleotides to silicon (1 0 0) surfaces in patterns is described. UV light exposure of silicon (1 0 0) coated with alkenes functionalized with carboxylic acid groups and derivatives results in covalent attachment to the silicon surface. The carboxyl-terminated (and derivatives) surfaces act as a substrate for the chemistry of the subsequent attachment of oligonucleotides. Illustrated are our results gained for optimisation of the surface attachment chemistry and the characterisation of the surface with scanning electron microscopy, epifluorescence microscopy and ellipsometry. © 2004 Elsevier B.V. All rights reserved. |
| spellingShingle | Yin, H Brown, T Greef, R Wilkinson, J Melvin, T Chemical modification and micropatterning of Si(100) with oligonucleotides |
| title | Chemical modification and micropatterning of Si(100) with oligonucleotides |
| title_full | Chemical modification and micropatterning of Si(100) with oligonucleotides |
| title_fullStr | Chemical modification and micropatterning of Si(100) with oligonucleotides |
| title_full_unstemmed | Chemical modification and micropatterning of Si(100) with oligonucleotides |
| title_short | Chemical modification and micropatterning of Si(100) with oligonucleotides |
| title_sort | chemical modification and micropatterning of si 100 with oligonucleotides |
| work_keys_str_mv | AT yinh chemicalmodificationandmicropatterningofsi100witholigonucleotides AT brownt chemicalmodificationandmicropatterningofsi100witholigonucleotides AT greefr chemicalmodificationandmicropatterningofsi100witholigonucleotides AT wilkinsonj chemicalmodificationandmicropatterningofsi100witholigonucleotides AT melvint chemicalmodificationandmicropatterningofsi100witholigonucleotides |