Proteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures.
Stationary-phase, carbon-starved shake-flask cultures of Saccharomyces cerevisiae are popular models for studying eukaryotic chronological aging. However, their nutrient-starved physiological status differs substantially from that of postmitotic metazoan cells. Retentostat cultures offer an attracti...
| Autori principali: | , , , , , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
American Chemical Society
2014
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| _version_ | 1826256639097831424 |
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| author | Binai, N Bisschops, M van Breukelen, B Mohammed, S Loeff, L Pronk, J Heck, A Daran-Lapujade, P Slijper, M |
| author_facet | Binai, N Bisschops, M van Breukelen, B Mohammed, S Loeff, L Pronk, J Heck, A Daran-Lapujade, P Slijper, M |
| author_sort | Binai, N |
| collection | OXFORD |
| description | Stationary-phase, carbon-starved shake-flask cultures of Saccharomyces cerevisiae are popular models for studying eukaryotic chronological aging. However, their nutrient-starved physiological status differs substantially from that of postmitotic metazoan cells. Retentostat cultures offer an attractive alternative model system in which yeast cells, maintained under continuous calorie restriction, hardly divide but retain high metabolic activity and viability for prolonged periods of time. Using TMT labeling and UHPLC-MS/MS, the present study explores the proteome of yeast cultures during transition from exponential growth to near-zero growth in severely calorie-restricted retentostats. This transition elicited protein level changes in 20% of the yeast proteome. Increased abundance of heat shock-related proteins correlated with increased transcript levels of the corresponding genes and was consistent with a strongly increased heat shock tolerance of retentostat-grown cells. A sizable fraction (43%) of the proteins with increased abundance under calorie restriction was involved in oxidative phosphorylation and in various mitochondrial functions that, under the anaerobic, nongrowing conditions used, have a very limited role. Although it may seem surprising that yeast cells confronted with severe calorie restriction invest in the synthesis of proteins that, under those conditions, do not contribute to fitness, these responses may confer metabolic flexibility and thereby a selective advantage in fluctuating natural habitats. |
| first_indexed | 2024-03-06T18:05:25Z |
| format | Journal article |
| id | oxford-uuid:0143cd3f-bfdb-4bd8-b444-2aff1899c62c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:05:25Z |
| publishDate | 2014 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:0143cd3f-bfdb-4bd8-b444-2aff1899c62c2022-03-26T08:34:02ZProteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0143cd3f-bfdb-4bd8-b444-2aff1899c62cEnglishSymplectic Elements at OxfordAmerican Chemical Society2014Binai, NBisschops, Mvan Breukelen, BMohammed, SLoeff, LPronk, JHeck, ADaran-Lapujade, PSlijper, MStationary-phase, carbon-starved shake-flask cultures of Saccharomyces cerevisiae are popular models for studying eukaryotic chronological aging. However, their nutrient-starved physiological status differs substantially from that of postmitotic metazoan cells. Retentostat cultures offer an attractive alternative model system in which yeast cells, maintained under continuous calorie restriction, hardly divide but retain high metabolic activity and viability for prolonged periods of time. Using TMT labeling and UHPLC-MS/MS, the present study explores the proteome of yeast cultures during transition from exponential growth to near-zero growth in severely calorie-restricted retentostats. This transition elicited protein level changes in 20% of the yeast proteome. Increased abundance of heat shock-related proteins correlated with increased transcript levels of the corresponding genes and was consistent with a strongly increased heat shock tolerance of retentostat-grown cells. A sizable fraction (43%) of the proteins with increased abundance under calorie restriction was involved in oxidative phosphorylation and in various mitochondrial functions that, under the anaerobic, nongrowing conditions used, have a very limited role. Although it may seem surprising that yeast cells confronted with severe calorie restriction invest in the synthesis of proteins that, under those conditions, do not contribute to fitness, these responses may confer metabolic flexibility and thereby a selective advantage in fluctuating natural habitats. |
| spellingShingle | Binai, N Bisschops, M van Breukelen, B Mohammed, S Loeff, L Pronk, J Heck, A Daran-Lapujade, P Slijper, M Proteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures. |
| title | Proteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures. |
| title_full | Proteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures. |
| title_fullStr | Proteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures. |
| title_full_unstemmed | Proteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures. |
| title_short | Proteome adaptation of Saccharomyces cerevisiae to severe calorie restriction in Retentostat cultures. |
| title_sort | proteome adaptation of saccharomyces cerevisiae to severe calorie restriction in retentostat cultures |
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