Considerations and implications in the purification of extracellular vesicles -- a cautionary tale
Extracellular vesicles (EVs) are nano-sized particles constitutively released from cells into all biological fluids. Interestingly, these vesicles contain genetic cargoes including proteins, RNA and bioactive lipids that can be functionally delivered and affect recipient cells. As a result, there is...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Frontiers Media
2019
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| _version_ | 1797050501503647744 |
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| author | Lee, YXF Johansson, H Wood, MJA El Andaloussi, S |
| author_facet | Lee, YXF Johansson, H Wood, MJA El Andaloussi, S |
| author_sort | Lee, YXF |
| collection | OXFORD |
| description | Extracellular vesicles (EVs) are nano-sized particles constitutively released from cells into all biological fluids. Interestingly, these vesicles contain genetic cargoes including proteins, RNA and bioactive lipids that can be functionally delivered and affect recipient cells. As a result, there is growing interest in studying EVs in pathological conditions, including central nervous system (CNS)-related diseases, as EVs may be used for diagnostic purposes or as therapeutic agents. However, one major bottleneck is the need for better EV purification strategies when considering complex biological sources such as serum/protein-rich media or plasma. In this study, we have performed a systematic comparison study between the current gold-standard method: ultracentrifugation, to an alternative: size-exclusion chromatography (LC), using induced pluripotent stem cell (iPSC) derived complex media as a model system. We demonstrate that LC allows for derivation of purer EVs from iPSCs, which was previously impossible with the original UC method. Importantly, our study further highlights the various drawbacks when using the conventional UC approach that lead to misinterpretation of EV data. Lastly, we describe novel data on our iPSC-EVs; how they could relate to stem cell biology and discuss their potential use as EV therapeutics for CNS diseases. |
| first_indexed | 2024-03-06T18:06:06Z |
| format | Journal article |
| id | oxford-uuid:017a5c7d-3d4a-4321-82a8-2c21167ca3a4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:06:06Z |
| publishDate | 2019 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | oxford-uuid:017a5c7d-3d4a-4321-82a8-2c21167ca3a42022-03-26T08:35:12ZConsiderations and implications in the purification of extracellular vesicles -- a cautionary taleJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:017a5c7d-3d4a-4321-82a8-2c21167ca3a4EnglishSymplectic ElementsFrontiers Media2019Lee, YXFJohansson, HWood, MJAEl Andaloussi, SExtracellular vesicles (EVs) are nano-sized particles constitutively released from cells into all biological fluids. Interestingly, these vesicles contain genetic cargoes including proteins, RNA and bioactive lipids that can be functionally delivered and affect recipient cells. As a result, there is growing interest in studying EVs in pathological conditions, including central nervous system (CNS)-related diseases, as EVs may be used for diagnostic purposes or as therapeutic agents. However, one major bottleneck is the need for better EV purification strategies when considering complex biological sources such as serum/protein-rich media or plasma. In this study, we have performed a systematic comparison study between the current gold-standard method: ultracentrifugation, to an alternative: size-exclusion chromatography (LC), using induced pluripotent stem cell (iPSC) derived complex media as a model system. We demonstrate that LC allows for derivation of purer EVs from iPSCs, which was previously impossible with the original UC method. Importantly, our study further highlights the various drawbacks when using the conventional UC approach that lead to misinterpretation of EV data. Lastly, we describe novel data on our iPSC-EVs; how they could relate to stem cell biology and discuss their potential use as EV therapeutics for CNS diseases. |
| spellingShingle | Lee, YXF Johansson, H Wood, MJA El Andaloussi, S Considerations and implications in the purification of extracellular vesicles -- a cautionary tale |
| title | Considerations and implications in the purification of extracellular vesicles -- a cautionary tale |
| title_full | Considerations and implications in the purification of extracellular vesicles -- a cautionary tale |
| title_fullStr | Considerations and implications in the purification of extracellular vesicles -- a cautionary tale |
| title_full_unstemmed | Considerations and implications in the purification of extracellular vesicles -- a cautionary tale |
| title_short | Considerations and implications in the purification of extracellular vesicles -- a cautionary tale |
| title_sort | considerations and implications in the purification of extracellular vesicles a cautionary tale |
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