Use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues.
Anthocyanins in extracts from raspberries and blueberries were analyzed by reversed-phase HPLC coupled to a high-resolution Exactive Orbitrap mass spectrometer (HR-MS) with a resolution of 100,000, operated with an electrospray source in the positive ionization mode. As consumption of anthocyanin-ri...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2010
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| _version_ | 1826256852955955200 |
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| author | Mullen, W Larcombe, S Arnold, K Welchman, H Crozier, A |
| author_facet | Mullen, W Larcombe, S Arnold, K Welchman, H Crozier, A |
| author_sort | Mullen, W |
| collection | OXFORD |
| description | Anthocyanins in extracts from raspberries and blueberries were analyzed by reversed-phase HPLC coupled to a high-resolution Exactive Orbitrap mass spectrometer (HR-MS) with a resolution of 100,000, operated with an electrospray source in the positive ionization mode. As consumption of anthocyanin-rich berry extracts has been associated with improved cognitive function, brain extracts from European greenfinches ( Carduelis chloris ) that had been fed one blackberry daily for a period of 2 weeks were analyzed by both HPLC with traditional tandem MS in the selected reaction monitoring mode and HPLC-HR-MS. Cyanidin-3-O-glucoside was detected in the brain extracts by both methods, but because of its high level of selectivity, HR-MS was ca. 200-fold more sensitive. A further advantage of HR-MS is that unlike MS-SRM it enables both targeted and nontargeted compounds to be detected and much lower limits of detection are achieved without compromising the selectivity of the analysis. |
| first_indexed | 2024-03-06T18:08:51Z |
| format | Journal article |
| id | oxford-uuid:02514516-af6c-4d71-8206-5d18bd3c3bb1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:08:51Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:02514516-af6c-4d71-8206-5d18bd3c3bb12022-03-26T08:40:02ZUse of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:02514516-af6c-4d71-8206-5d18bd3c3bb1EnglishSymplectic Elements at Oxford2010Mullen, WLarcombe, SArnold, KWelchman, HCrozier, AAnthocyanins in extracts from raspberries and blueberries were analyzed by reversed-phase HPLC coupled to a high-resolution Exactive Orbitrap mass spectrometer (HR-MS) with a resolution of 100,000, operated with an electrospray source in the positive ionization mode. As consumption of anthocyanin-rich berry extracts has been associated with improved cognitive function, brain extracts from European greenfinches ( Carduelis chloris ) that had been fed one blackberry daily for a period of 2 weeks were analyzed by both HPLC with traditional tandem MS in the selected reaction monitoring mode and HPLC-HR-MS. Cyanidin-3-O-glucoside was detected in the brain extracts by both methods, but because of its high level of selectivity, HR-MS was ca. 200-fold more sensitive. A further advantage of HR-MS is that unlike MS-SRM it enables both targeted and nontargeted compounds to be detected and much lower limits of detection are achieved without compromising the selectivity of the analysis. |
| spellingShingle | Mullen, W Larcombe, S Arnold, K Welchman, H Crozier, A Use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues. |
| title | Use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues. |
| title_full | Use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues. |
| title_fullStr | Use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues. |
| title_full_unstemmed | Use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues. |
| title_short | Use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues. |
| title_sort | use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry fed tissues |
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