S1 sensitive sites in adenovirus DNA.
S1 nuclease has been used as a probe for regions of DNA secondary structure in supercoiled recombinant plasmids containing adenovirus (Ad) DNA sequences. In the sequences examined two S1 sensitive sites were identified in the left-terminal 16.5% of Ad 12 DNA, one of which aligned approximately with...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1983
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| _version_ | 1826256872986902528 |
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| author | Goding, C Russell, W |
| author_facet | Goding, C Russell, W |
| author_sort | Goding, C |
| collection | OXFORD |
| description | S1 nuclease has been used as a probe for regions of DNA secondary structure in supercoiled recombinant plasmids containing adenovirus (Ad) DNA sequences. In the sequences examined two S1 sensitive sites were identified in the left-terminal 16.5% of Ad 12 DNA, one of which aligned approximately with an inverted repeat region in the DNA sequence. In addition an S1 sensitive site was dictated by a potential cruciform structure in the region of the Ad 2 major late promoter. In contrast to the expected cleavage site at the loop of the cruciform, cleavage occurred at the base of the stem in the region of the TATA box. All three S1 sensitive sites identified were more sensitive to S1 than the endogenous sites in the parent plasmids. |
| first_indexed | 2024-03-06T18:09:11Z |
| format | Journal article |
| id | oxford-uuid:0268323a-3eb1-4c06-b747-2fa791547b80 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:09:11Z |
| publishDate | 1983 |
| record_format | dspace |
| spelling | oxford-uuid:0268323a-3eb1-4c06-b747-2fa791547b802022-03-26T08:40:39ZS1 sensitive sites in adenovirus DNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0268323a-3eb1-4c06-b747-2fa791547b80EnglishSymplectic Elements at Oxford1983Goding, CRussell, WS1 nuclease has been used as a probe for regions of DNA secondary structure in supercoiled recombinant plasmids containing adenovirus (Ad) DNA sequences. In the sequences examined two S1 sensitive sites were identified in the left-terminal 16.5% of Ad 12 DNA, one of which aligned approximately with an inverted repeat region in the DNA sequence. In addition an S1 sensitive site was dictated by a potential cruciform structure in the region of the Ad 2 major late promoter. In contrast to the expected cleavage site at the loop of the cruciform, cleavage occurred at the base of the stem in the region of the TATA box. All three S1 sensitive sites identified were more sensitive to S1 than the endogenous sites in the parent plasmids. |
| spellingShingle | Goding, C Russell, W S1 sensitive sites in adenovirus DNA. |
| title | S1 sensitive sites in adenovirus DNA. |
| title_full | S1 sensitive sites in adenovirus DNA. |
| title_fullStr | S1 sensitive sites in adenovirus DNA. |
| title_full_unstemmed | S1 sensitive sites in adenovirus DNA. |
| title_short | S1 sensitive sites in adenovirus DNA. |
| title_sort | s1 sensitive sites in adenovirus dna |
| work_keys_str_mv | AT godingc s1sensitivesitesinadenovirusdna AT russellw s1sensitivesitesinadenovirusdna |