Antibodies to aquaporin-1 are not present in neuromyelitis optica
Although more than 70% of all patients with neuromyelitis optica (NMO) are seropositive for aquaporin-4 (AQP4) antibodies, a substantial proportion of patients fulfilling the clinical criteria for NMO or limited forms of the disease (NMO spectrum disorders [NMOSD]) are negative for these antibodies....
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Format: | Journal article |
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American Academy of Neurology
2015
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author | Waters, P Leite, M Palace, J |
author_facet | Waters, P Leite, M Palace, J |
author_sort | Waters, P |
collection | OXFORD |
description | Although more than 70% of all patients with neuromyelitis optica (NMO) are seropositive for aquaporin-4 (AQP4) antibodies, a substantial proportion of patients fulfilling the clinical criteria for NMO or limited forms of the disease (NMO spectrum disorders [NMOSD]) are negative for these antibodies.1 This raises the possibility of other autoantibodies in these patients. One putative target, the ubiquitously expressed water channel aquaporin-1 (AQP1), which is partially lost or internalized in certain NMO lesions,2 was described in a subset of patients with NMOSD.3,–,5 However, AQP1 antibodies were also found in patients with multiple sclerosis (MS), thus raising concerns about the specificity of these findings. Therefore, we developed a recombinant live cell immunofluorescence assay (CBA) for AQP1 antibodies based on our AQP4 antibody assay.6 We analyzed 176 serum samples from Austrian patients with NMOSD (n = 67), Austrian patients with MS (n = 31), and controls (n = 78) for the presence of IgG AQP1 antibodies and AQP4 antibodies. Furthermore, both assays were validated in a blinded cohort of 58 patients with NMOSD (n = 36) or MS (n = 22) from Lyon (France) and Oxford (United Kingdom). Clinical and demographic data of patients and controls are shown in the table. |
first_indexed | 2024-03-06T18:10:34Z |
format | Journal article |
id | oxford-uuid:02e0fe6e-509e-44af-9c23-92464e36cdf4 |
institution | University of Oxford |
last_indexed | 2024-03-06T18:10:34Z |
publishDate | 2015 |
publisher | American Academy of Neurology |
record_format | dspace |
spelling | oxford-uuid:02e0fe6e-509e-44af-9c23-92464e36cdf42022-03-26T08:43:10ZAntibodies to aquaporin-1 are not present in neuromyelitis opticaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:02e0fe6e-509e-44af-9c23-92464e36cdf4Symplectic Elements at OxfordAmerican Academy of Neurology2015Waters, PLeite, MPalace, JAlthough more than 70% of all patients with neuromyelitis optica (NMO) are seropositive for aquaporin-4 (AQP4) antibodies, a substantial proportion of patients fulfilling the clinical criteria for NMO or limited forms of the disease (NMO spectrum disorders [NMOSD]) are negative for these antibodies.1 This raises the possibility of other autoantibodies in these patients. One putative target, the ubiquitously expressed water channel aquaporin-1 (AQP1), which is partially lost or internalized in certain NMO lesions,2 was described in a subset of patients with NMOSD.3,–,5 However, AQP1 antibodies were also found in patients with multiple sclerosis (MS), thus raising concerns about the specificity of these findings. Therefore, we developed a recombinant live cell immunofluorescence assay (CBA) for AQP1 antibodies based on our AQP4 antibody assay.6 We analyzed 176 serum samples from Austrian patients with NMOSD (n = 67), Austrian patients with MS (n = 31), and controls (n = 78) for the presence of IgG AQP1 antibodies and AQP4 antibodies. Furthermore, both assays were validated in a blinded cohort of 58 patients with NMOSD (n = 36) or MS (n = 22) from Lyon (France) and Oxford (United Kingdom). Clinical and demographic data of patients and controls are shown in the table. |
spellingShingle | Waters, P Leite, M Palace, J Antibodies to aquaporin-1 are not present in neuromyelitis optica |
title | Antibodies to aquaporin-1 are not present in neuromyelitis optica |
title_full | Antibodies to aquaporin-1 are not present in neuromyelitis optica |
title_fullStr | Antibodies to aquaporin-1 are not present in neuromyelitis optica |
title_full_unstemmed | Antibodies to aquaporin-1 are not present in neuromyelitis optica |
title_short | Antibodies to aquaporin-1 are not present in neuromyelitis optica |
title_sort | antibodies to aquaporin 1 are not present in neuromyelitis optica |
work_keys_str_mv | AT watersp antibodiestoaquaporin1arenotpresentinneuromyelitisoptica AT leitem antibodiestoaquaporin1arenotpresentinneuromyelitisoptica AT palacej antibodiestoaquaporin1arenotpresentinneuromyelitisoptica |