Antibodies to aquaporin-1 are not present in neuromyelitis optica

Although more than 70% of all patients with neuromyelitis optica (NMO) are seropositive for aquaporin-4 (AQP4) antibodies, a substantial proportion of patients fulfilling the clinical criteria for NMO or limited forms of the disease (NMO spectrum disorders [NMOSD]) are negative for these antibodies....

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Main Authors: Waters, P, Leite, M, Palace, J
Format: Journal article
Published: American Academy of Neurology 2015
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author Waters, P
Leite, M
Palace, J
author_facet Waters, P
Leite, M
Palace, J
author_sort Waters, P
collection OXFORD
description Although more than 70% of all patients with neuromyelitis optica (NMO) are seropositive for aquaporin-4 (AQP4) antibodies, a substantial proportion of patients fulfilling the clinical criteria for NMO or limited forms of the disease (NMO spectrum disorders [NMOSD]) are negative for these antibodies.1 This raises the possibility of other autoantibodies in these patients. One putative target, the ubiquitously expressed water channel aquaporin-1 (AQP1), which is partially lost or internalized in certain NMO lesions,2 was described in a subset of patients with NMOSD.3,–,5 However, AQP1 antibodies were also found in patients with multiple sclerosis (MS), thus raising concerns about the specificity of these findings. Therefore, we developed a recombinant live cell immunofluorescence assay (CBA) for AQP1 antibodies based on our AQP4 antibody assay.6 We analyzed 176 serum samples from Austrian patients with NMOSD (n = 67), Austrian patients with MS (n = 31), and controls (n = 78) for the presence of IgG AQP1 antibodies and AQP4 antibodies. Furthermore, both assays were validated in a blinded cohort of 58 patients with NMOSD (n = 36) or MS (n = 22) from Lyon (France) and Oxford (United Kingdom). Clinical and demographic data of patients and controls are shown in the table.
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spelling oxford-uuid:02e0fe6e-509e-44af-9c23-92464e36cdf42022-03-26T08:43:10ZAntibodies to aquaporin-1 are not present in neuromyelitis opticaJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:02e0fe6e-509e-44af-9c23-92464e36cdf4Symplectic Elements at OxfordAmerican Academy of Neurology2015Waters, PLeite, MPalace, JAlthough more than 70% of all patients with neuromyelitis optica (NMO) are seropositive for aquaporin-4 (AQP4) antibodies, a substantial proportion of patients fulfilling the clinical criteria for NMO or limited forms of the disease (NMO spectrum disorders [NMOSD]) are negative for these antibodies.1 This raises the possibility of other autoantibodies in these patients. One putative target, the ubiquitously expressed water channel aquaporin-1 (AQP1), which is partially lost or internalized in certain NMO lesions,2 was described in a subset of patients with NMOSD.3,–,5 However, AQP1 antibodies were also found in patients with multiple sclerosis (MS), thus raising concerns about the specificity of these findings. Therefore, we developed a recombinant live cell immunofluorescence assay (CBA) for AQP1 antibodies based on our AQP4 antibody assay.6 We analyzed 176 serum samples from Austrian patients with NMOSD (n = 67), Austrian patients with MS (n = 31), and controls (n = 78) for the presence of IgG AQP1 antibodies and AQP4 antibodies. Furthermore, both assays were validated in a blinded cohort of 58 patients with NMOSD (n = 36) or MS (n = 22) from Lyon (France) and Oxford (United Kingdom). Clinical and demographic data of patients and controls are shown in the table.
spellingShingle Waters, P
Leite, M
Palace, J
Antibodies to aquaporin-1 are not present in neuromyelitis optica
title Antibodies to aquaporin-1 are not present in neuromyelitis optica
title_full Antibodies to aquaporin-1 are not present in neuromyelitis optica
title_fullStr Antibodies to aquaporin-1 are not present in neuromyelitis optica
title_full_unstemmed Antibodies to aquaporin-1 are not present in neuromyelitis optica
title_short Antibodies to aquaporin-1 are not present in neuromyelitis optica
title_sort antibodies to aquaporin 1 are not present in neuromyelitis optica
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AT leitem antibodiestoaquaporin1arenotpresentinneuromyelitisoptica
AT palacej antibodiestoaquaporin1arenotpresentinneuromyelitisoptica