Mass spectrometry: Come of age for structural and dynamical biology
Over the past two decades, mass spectrometry (MS) has emerged as a bone fide approach for structural biology. MS can inform on all levels of protein organization, and enables quantitative assessments of their intrinsic dynamics. The key advantages of MS are that it is a sensitive, high-resolution se...
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| Format: | Journal article |
| Language: | English |
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2011
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| _version_ | 1826256985685753856 |
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| author | Benesch, J Ruotolo, B |
| author_facet | Benesch, J Ruotolo, B |
| author_sort | Benesch, J |
| collection | OXFORD |
| description | Over the past two decades, mass spectrometry (MS) has emerged as a bone fide approach for structural biology. MS can inform on all levels of protein organization, and enables quantitative assessments of their intrinsic dynamics. The key advantages of MS are that it is a sensitive, high-resolution separation technique with wide applicability, and thereby allows the interrogation of transient protein assemblies in the context of complex mixtures. Here we describe how molecular-level information is derived from MS experiments, and how it can be combined with spatial and dynamical restraints obtained from other structural biology approaches to allow hybrid studies of protein architecture and movements. © 2011 Elsevier Ltd. |
| first_indexed | 2024-03-06T18:10:58Z |
| format | Journal article |
| id | oxford-uuid:03021ddf-5a87-4502-8ec3-c8590357305f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:10:58Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:03021ddf-5a87-4502-8ec3-c8590357305f2022-03-26T08:43:49ZMass spectrometry: Come of age for structural and dynamical biologyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:03021ddf-5a87-4502-8ec3-c8590357305fEnglishSymplectic Elements at Oxford2011Benesch, JRuotolo, BOver the past two decades, mass spectrometry (MS) has emerged as a bone fide approach for structural biology. MS can inform on all levels of protein organization, and enables quantitative assessments of their intrinsic dynamics. The key advantages of MS are that it is a sensitive, high-resolution separation technique with wide applicability, and thereby allows the interrogation of transient protein assemblies in the context of complex mixtures. Here we describe how molecular-level information is derived from MS experiments, and how it can be combined with spatial and dynamical restraints obtained from other structural biology approaches to allow hybrid studies of protein architecture and movements. © 2011 Elsevier Ltd. |
| spellingShingle | Benesch, J Ruotolo, B Mass spectrometry: Come of age for structural and dynamical biology |
| title | Mass spectrometry: Come of age for structural and dynamical biology |
| title_full | Mass spectrometry: Come of age for structural and dynamical biology |
| title_fullStr | Mass spectrometry: Come of age for structural and dynamical biology |
| title_full_unstemmed | Mass spectrometry: Come of age for structural and dynamical biology |
| title_short | Mass spectrometry: Come of age for structural and dynamical biology |
| title_sort | mass spectrometry come of age for structural and dynamical biology |
| work_keys_str_mv | AT beneschj massspectrometrycomeofageforstructuralanddynamicalbiology AT ruotolob massspectrometrycomeofageforstructuralanddynamicalbiology |