Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry.
The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free c...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2007
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