In situ structure of an intact lipopolysaccharide-bound bacterial surface layer
Most bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Cau...
| Những tác giả chính: | , , , , , , , |
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| Định dạng: | Journal article |
| Ngôn ngữ: | English |
| Được phát hành: |
Elsevier
2019
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| _version_ | 1826257163717181440 |
|---|---|
| author | Von Kügelgen, A Tang, H Hardy, GG Kureisaite-Ciziene, D Brun, YV Stansfeld, PJ Robinson, CV Bharat, TAM |
| author_facet | Von Kügelgen, A Tang, H Hardy, GG Kureisaite-Ciziene, D Brun, YV Stansfeld, PJ Robinson, CV Bharat, TAM |
| author_sort | Von Kügelgen, A |
| collection | OXFORD |
| description | Most bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide. Using native mass spectrometry and molecular dynamics simulations, we deduce the length of the O-antigen on cells and show how lipopolysaccharide binding and S-layer assembly is regulated by calcium. Finally, we present a near-atomic resolution in situ structure of the complete S-layer using cellular electron cryotomography, showing S-layer arrangement at the tip of the O-antigen. A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications. |
| first_indexed | 2024-03-06T18:13:46Z |
| format | Journal article |
| id | oxford-uuid:03e58867-3eab-4c6a-a83c-72078889c5af |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:13:46Z |
| publishDate | 2019 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:03e58867-3eab-4c6a-a83c-72078889c5af2022-03-26T08:48:53ZIn situ structure of an intact lipopolysaccharide-bound bacterial surface layerJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:03e58867-3eab-4c6a-a83c-72078889c5afEnglishSymplectic Elements at OxfordElsevier2019Von Kügelgen, ATang, HHardy, GGKureisaite-Ciziene, DBrun, YVStansfeld, PJRobinson, CVBharat, TAMMost bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide. Using native mass spectrometry and molecular dynamics simulations, we deduce the length of the O-antigen on cells and show how lipopolysaccharide binding and S-layer assembly is regulated by calcium. Finally, we present a near-atomic resolution in situ structure of the complete S-layer using cellular electron cryotomography, showing S-layer arrangement at the tip of the O-antigen. A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications. |
| spellingShingle | Von Kügelgen, A Tang, H Hardy, GG Kureisaite-Ciziene, D Brun, YV Stansfeld, PJ Robinson, CV Bharat, TAM In situ structure of an intact lipopolysaccharide-bound bacterial surface layer |
| title | In situ structure of an intact lipopolysaccharide-bound bacterial surface layer |
| title_full | In situ structure of an intact lipopolysaccharide-bound bacterial surface layer |
| title_fullStr | In situ structure of an intact lipopolysaccharide-bound bacterial surface layer |
| title_full_unstemmed | In situ structure of an intact lipopolysaccharide-bound bacterial surface layer |
| title_short | In situ structure of an intact lipopolysaccharide-bound bacterial surface layer |
| title_sort | in situ structure of an intact lipopolysaccharide bound bacterial surface layer |
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