Breast milk cellular HIV-specific interferon γ responses are associated with protection from peripartum HIV transmission.
OBJECTIVE: Breast milk is a major route of infant HIV infection, yet the majority of breast-fed, HIV-exposed infants escape infection by unknown mechanisms. This study aimed to investigate the role of HIV-specific breast milk cells in preventing infant HIV infection. DESIGN: A prospective study was...
| প্রধান লেখক: | , , , , , , , , , , |
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| বিন্যাস: | Journal article |
| ভাষা: | English |
| প্রকাশিত: |
2012
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| _version_ | 1826257314327298048 |
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| author | Lohman-Payne, B Slyker, J Moore, S Maleche-Obimbo, E Wamalwa, D Richardson, B Rowland-Jones, S Mbori-Ngacha, D Farquhar, C Overbaugh, J John-Stewart, G |
| author_facet | Lohman-Payne, B Slyker, J Moore, S Maleche-Obimbo, E Wamalwa, D Richardson, B Rowland-Jones, S Mbori-Ngacha, D Farquhar, C Overbaugh, J John-Stewart, G |
| author_sort | Lohman-Payne, B |
| collection | OXFORD |
| description | OBJECTIVE: Breast milk is a major route of infant HIV infection, yet the majority of breast-fed, HIV-exposed infants escape infection by unknown mechanisms. This study aimed to investigate the role of HIV-specific breast milk cells in preventing infant HIV infection. DESIGN: A prospective study was designed to measure associations between maternal breast milk HIV-specific interferon-γ (IFN-γ) responses and infant HIV-1 detection at 1 month of age. METHODS: In a Kenyan cohort of HIV-infected mothers, blood and breast milk HIV-gag IFN-γ ELISpot responses were measured. Logistic regression was used to measure associations between breast milk IFN-γ responses and infant HIV infection at 1 month of age. RESULTS: IFN-γ responses were detected in breast milk from 117 of 170 (69%) women. IFN-γ responses were associated with breast milk viral load, levels of macrophage inflammatory protein (MIP) 1α, MIP-1β, regulated upon activation, normal T-cell expressed, and secreted and stromal-cell derived factor 1 and subclinical mastitis. Univariate factors associated with infant HIV infection at 1 month postpartum included both detection and breadth of breast milk IFN-γ response (P = 0.08, P = 0.04, respectively), breast milk MIP-1β detection (P = 0.05), and plasma (P = 0.004) and breast milk (P = 0.004) viral load. In multivariate analyses adjusting for breast milk viral load and MIP-1β, breast milk IFN-γ responses were associated with an approximately 70% reduction in infant HIV infection [adjusted odds ratio (aOR) 0.29, 95% confidence interval (CI) 0.092-0.91], and each additional peptide pool targeted was associated with an approximately 35% reduction in infant HIV (aOR 0.65, 95% CI 0.44-0.97). CONCLUSION: These data show breast milk HIV-gag-specific IFN-γ cellular immune responses are prevalent and may contribute to protection from early HIV transmission. More broadly, these data suggest breast milk cellular responses are potentially influential in decreasing mother-to-child transmission of viruses. |
| first_indexed | 2024-03-06T18:16:12Z |
| format | Journal article |
| id | oxford-uuid:04b4babf-e6e7-4d3e-a7d6-4b4994a2c38d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:16:12Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:04b4babf-e6e7-4d3e-a7d6-4b4994a2c38d2022-03-26T08:53:12ZBreast milk cellular HIV-specific interferon γ responses are associated with protection from peripartum HIV transmission.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:04b4babf-e6e7-4d3e-a7d6-4b4994a2c38dEnglishSymplectic Elements at Oxford2012Lohman-Payne, BSlyker, JMoore, SMaleche-Obimbo, EWamalwa, DRichardson, BRowland-Jones, SMbori-Ngacha, DFarquhar, COverbaugh, JJohn-Stewart, GOBJECTIVE: Breast milk is a major route of infant HIV infection, yet the majority of breast-fed, HIV-exposed infants escape infection by unknown mechanisms. This study aimed to investigate the role of HIV-specific breast milk cells in preventing infant HIV infection. DESIGN: A prospective study was designed to measure associations between maternal breast milk HIV-specific interferon-γ (IFN-γ) responses and infant HIV-1 detection at 1 month of age. METHODS: In a Kenyan cohort of HIV-infected mothers, blood and breast milk HIV-gag IFN-γ ELISpot responses were measured. Logistic regression was used to measure associations between breast milk IFN-γ responses and infant HIV infection at 1 month of age. RESULTS: IFN-γ responses were detected in breast milk from 117 of 170 (69%) women. IFN-γ responses were associated with breast milk viral load, levels of macrophage inflammatory protein (MIP) 1α, MIP-1β, regulated upon activation, normal T-cell expressed, and secreted and stromal-cell derived factor 1 and subclinical mastitis. Univariate factors associated with infant HIV infection at 1 month postpartum included both detection and breadth of breast milk IFN-γ response (P = 0.08, P = 0.04, respectively), breast milk MIP-1β detection (P = 0.05), and plasma (P = 0.004) and breast milk (P = 0.004) viral load. In multivariate analyses adjusting for breast milk viral load and MIP-1β, breast milk IFN-γ responses were associated with an approximately 70% reduction in infant HIV infection [adjusted odds ratio (aOR) 0.29, 95% confidence interval (CI) 0.092-0.91], and each additional peptide pool targeted was associated with an approximately 35% reduction in infant HIV (aOR 0.65, 95% CI 0.44-0.97). CONCLUSION: These data show breast milk HIV-gag-specific IFN-γ cellular immune responses are prevalent and may contribute to protection from early HIV transmission. More broadly, these data suggest breast milk cellular responses are potentially influential in decreasing mother-to-child transmission of viruses. |
| spellingShingle | Lohman-Payne, B Slyker, J Moore, S Maleche-Obimbo, E Wamalwa, D Richardson, B Rowland-Jones, S Mbori-Ngacha, D Farquhar, C Overbaugh, J John-Stewart, G Breast milk cellular HIV-specific interferon γ responses are associated with protection from peripartum HIV transmission. |
| title | Breast milk cellular HIV-specific interferon γ responses are associated with protection from peripartum HIV transmission. |
| title_full | Breast milk cellular HIV-specific interferon γ responses are associated with protection from peripartum HIV transmission. |
| title_fullStr | Breast milk cellular HIV-specific interferon γ responses are associated with protection from peripartum HIV transmission. |
| title_full_unstemmed | Breast milk cellular HIV-specific interferon γ responses are associated with protection from peripartum HIV transmission. |
| title_short | Breast milk cellular HIV-specific interferon γ responses are associated with protection from peripartum HIV transmission. |
| title_sort | breast milk cellular hiv specific interferon γ responses are associated with protection from peripartum hiv transmission |
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