Identifying chondrogenesis strategies for tissue engineering of articular cartilage
A key step in the tissue engineering of articular cartilage is the chondrogenic differentiation of mesenchymal stem cells (MSCs) into chondrocytes (native cartilage cells). Chondrogenesis is regulated by transforming growth factor-β (TGF-β), a short-lived cytokine whose effect is prolonged by storag...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Published: |
SAGE Publications
2019
|
| _version_ | 1826257440744669184 |
|---|---|
| author | Chen, M Whiteley, J Please, C Ehlicke, F Waters, S Byrne, H |
| author_facet | Chen, M Whiteley, J Please, C Ehlicke, F Waters, S Byrne, H |
| author_sort | Chen, M |
| collection | OXFORD |
| description | A key step in the tissue engineering of articular cartilage is the chondrogenic differentiation of mesenchymal stem cells (MSCs) into chondrocytes (native cartilage cells). Chondrogenesis is regulated by transforming growth factor-β (TGF-β), a short-lived cytokine whose effect is prolonged by storage in the extracellular matrix. Tissue engineering applications aim to maximise the yield of differentiated MSCs. Recent experiments involve seeding a hydrogel construct with a layer of MSCs lying below a layer of chondrocytes, stimulating the seeded cells in the construct from above with exogenous TGF-β and then culturing it in vitro. To investigate the efficacy of this strategy, we develop a mathematical model to describe the interactions between MSCs, chondrocytes and TGF-β. Using this model, we investigate the effect of varying the initial concentration of TGF-β, the initial densities of the MSCs and chondrocytes, and the relative depths of the two layers on the long-time composition of the tissue construct. |
| first_indexed | 2024-03-06T18:18:14Z |
| format | Journal article |
| id | oxford-uuid:05606e0e-e4b9-4fff-acd1-9a2e6e393165 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:18:14Z |
| publishDate | 2019 |
| publisher | SAGE Publications |
| record_format | dspace |
| spelling | oxford-uuid:05606e0e-e4b9-4fff-acd1-9a2e6e3931652022-03-26T08:56:50ZIdentifying chondrogenesis strategies for tissue engineering of articular cartilageJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:05606e0e-e4b9-4fff-acd1-9a2e6e393165Symplectic Elements at OxfordSAGE Publications2019Chen, MWhiteley, JPlease, CEhlicke, FWaters, SByrne, HA key step in the tissue engineering of articular cartilage is the chondrogenic differentiation of mesenchymal stem cells (MSCs) into chondrocytes (native cartilage cells). Chondrogenesis is regulated by transforming growth factor-β (TGF-β), a short-lived cytokine whose effect is prolonged by storage in the extracellular matrix. Tissue engineering applications aim to maximise the yield of differentiated MSCs. Recent experiments involve seeding a hydrogel construct with a layer of MSCs lying below a layer of chondrocytes, stimulating the seeded cells in the construct from above with exogenous TGF-β and then culturing it in vitro. To investigate the efficacy of this strategy, we develop a mathematical model to describe the interactions between MSCs, chondrocytes and TGF-β. Using this model, we investigate the effect of varying the initial concentration of TGF-β, the initial densities of the MSCs and chondrocytes, and the relative depths of the two layers on the long-time composition of the tissue construct. |
| spellingShingle | Chen, M Whiteley, J Please, C Ehlicke, F Waters, S Byrne, H Identifying chondrogenesis strategies for tissue engineering of articular cartilage |
| title | Identifying chondrogenesis strategies for tissue engineering of articular cartilage |
| title_full | Identifying chondrogenesis strategies for tissue engineering of articular cartilage |
| title_fullStr | Identifying chondrogenesis strategies for tissue engineering of articular cartilage |
| title_full_unstemmed | Identifying chondrogenesis strategies for tissue engineering of articular cartilage |
| title_short | Identifying chondrogenesis strategies for tissue engineering of articular cartilage |
| title_sort | identifying chondrogenesis strategies for tissue engineering of articular cartilage |
| work_keys_str_mv | AT chenm identifyingchondrogenesisstrategiesfortissueengineeringofarticularcartilage AT whiteleyj identifyingchondrogenesisstrategiesfortissueengineeringofarticularcartilage AT pleasec identifyingchondrogenesisstrategiesfortissueengineeringofarticularcartilage AT ehlickef identifyingchondrogenesisstrategiesfortissueengineeringofarticularcartilage AT waterss identifyingchondrogenesisstrategiesfortissueengineeringofarticularcartilage AT byrneh identifyingchondrogenesisstrategiesfortissueengineeringofarticularcartilage |