Engineering entropy-driven reactions and networks catalyzed by DNA.
Artificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has...
| المؤلفون الرئيسيون: | , , , |
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| التنسيق: | Journal article |
| اللغة: | English |
| منشور في: |
2007
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| _version_ | 1826257468018130944 |
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| author | Zhang, D Turberfield, A Yurke, B Winfree, E |
| author_facet | Zhang, D Turberfield, A Yurke, B Winfree, E |
| author_sort | Zhang, D |
| collection | OXFORD |
| description | Artificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has been difficult to incorporate signal amplification components. We introduce a design strategy that allows a specified input oligonucleotide to catalyze the release of a specified output oligonucleotide, which in turn can serve as a catalyst for other reactions. This reaction, which is driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. We have constructed and characterized several circuits that amplify nucleic acid signals, including a feedforward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics. |
| first_indexed | 2024-03-06T18:18:41Z |
| format | Journal article |
| id | oxford-uuid:0585f95e-97f7-4567-8c03-d3a8ff3b888f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:18:41Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:0585f95e-97f7-4567-8c03-d3a8ff3b888f2022-03-26T08:57:35ZEngineering entropy-driven reactions and networks catalyzed by DNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0585f95e-97f7-4567-8c03-d3a8ff3b888fEnglishSymplectic Elements at Oxford2007Zhang, DTurberfield, AYurke, BWinfree, EArtificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has been difficult to incorporate signal amplification components. We introduce a design strategy that allows a specified input oligonucleotide to catalyze the release of a specified output oligonucleotide, which in turn can serve as a catalyst for other reactions. This reaction, which is driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. We have constructed and characterized several circuits that amplify nucleic acid signals, including a feedforward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics. |
| spellingShingle | Zhang, D Turberfield, A Yurke, B Winfree, E Engineering entropy-driven reactions and networks catalyzed by DNA. |
| title | Engineering entropy-driven reactions and networks catalyzed by DNA. |
| title_full | Engineering entropy-driven reactions and networks catalyzed by DNA. |
| title_fullStr | Engineering entropy-driven reactions and networks catalyzed by DNA. |
| title_full_unstemmed | Engineering entropy-driven reactions and networks catalyzed by DNA. |
| title_short | Engineering entropy-driven reactions and networks catalyzed by DNA. |
| title_sort | engineering entropy driven reactions and networks catalyzed by dna |
| work_keys_str_mv | AT zhangd engineeringentropydrivenreactionsandnetworkscatalyzedbydna AT turberfielda engineeringentropydrivenreactionsandnetworkscatalyzedbydna AT yurkeb engineeringentropydrivenreactionsandnetworkscatalyzedbydna AT winfreee engineeringentropydrivenreactionsandnetworkscatalyzedbydna |