Engineering entropy-driven reactions and networks catalyzed by DNA.

Artificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has...

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Egile Nagusiak: Zhang, D, Turberfield, A, Yurke, B, Winfree, E
Formatua: Journal article
Hizkuntza:English
Argitaratua: 2007
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author Zhang, D
Turberfield, A
Yurke, B
Winfree, E
author_facet Zhang, D
Turberfield, A
Yurke, B
Winfree, E
author_sort Zhang, D
collection OXFORD
description Artificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has been difficult to incorporate signal amplification components. We introduce a design strategy that allows a specified input oligonucleotide to catalyze the release of a specified output oligonucleotide, which in turn can serve as a catalyst for other reactions. This reaction, which is driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. We have constructed and characterized several circuits that amplify nucleic acid signals, including a feedforward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics.
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spelling oxford-uuid:0585f95e-97f7-4567-8c03-d3a8ff3b888f2022-03-26T08:57:35ZEngineering entropy-driven reactions and networks catalyzed by DNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0585f95e-97f7-4567-8c03-d3a8ff3b888fEnglishSymplectic Elements at Oxford2007Zhang, DTurberfield, AYurke, BWinfree, EArtificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has been difficult to incorporate signal amplification components. We introduce a design strategy that allows a specified input oligonucleotide to catalyze the release of a specified output oligonucleotide, which in turn can serve as a catalyst for other reactions. This reaction, which is driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. We have constructed and characterized several circuits that amplify nucleic acid signals, including a feedforward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics.
spellingShingle Zhang, D
Turberfield, A
Yurke, B
Winfree, E
Engineering entropy-driven reactions and networks catalyzed by DNA.
title Engineering entropy-driven reactions and networks catalyzed by DNA.
title_full Engineering entropy-driven reactions and networks catalyzed by DNA.
title_fullStr Engineering entropy-driven reactions and networks catalyzed by DNA.
title_full_unstemmed Engineering entropy-driven reactions and networks catalyzed by DNA.
title_short Engineering entropy-driven reactions and networks catalyzed by DNA.
title_sort engineering entropy driven reactions and networks catalyzed by dna
work_keys_str_mv AT zhangd engineeringentropydrivenreactionsandnetworkscatalyzedbydna
AT turberfielda engineeringentropydrivenreactionsandnetworkscatalyzedbydna
AT yurkeb engineeringentropydrivenreactionsandnetworkscatalyzedbydna
AT winfreee engineeringentropydrivenreactionsandnetworkscatalyzedbydna