Coupled metabolic cycles allow out-of-equilibrium autopoietic vesicle replication
We report chemically fuelled out‐of‐equilibrium self‐replicating vesicles based on surfactant formation. We studied the vesicles’ autocatalytic formation using UPLC to determine monomer concentration and interferometric scattering microscopy at the nanoparticle level. Unlike related reports of chemi...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Wiley
2020
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| _version_ | 1797051461908037632 |
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| author | Engwerda, AHJ Southworth, J Lebedeva, MA Scanes, RJH Kukura, P Fletcher, SP |
| author_facet | Engwerda, AHJ Southworth, J Lebedeva, MA Scanes, RJH Kukura, P Fletcher, SP |
| author_sort | Engwerda, AHJ |
| collection | OXFORD |
| description | We report chemically fuelled out‐of‐equilibrium self‐replicating vesicles based on surfactant formation. We studied the vesicles’ autocatalytic formation using UPLC to determine monomer concentration and interferometric scattering microscopy at the nanoparticle level. Unlike related reports of chemically fuelled self‐replicating micelles, our vesicular system was too stable to surfactant degradation to be maintained out of equilibrium. The introduction of a catalyst, which introduces a second catalytic cycle into the metabolic network, was used to close the first cycle. This shows how coupled catalytic cycles can create a metabolic network that allows the creation and perseverance of fuel‐driven, out‐of‐equilibrium self‐replicating vesicles. |
| first_indexed | 2024-03-06T18:19:51Z |
| format | Journal article |
| id | oxford-uuid:05e7319e-99a4-4553-ada3-99f1315cf278 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:19:51Z |
| publishDate | 2020 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:05e7319e-99a4-4553-ada3-99f1315cf2782022-03-26T08:59:42ZCoupled metabolic cycles allow out-of-equilibrium autopoietic vesicle replicationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:05e7319e-99a4-4553-ada3-99f1315cf278EnglishSymplectic ElementsWiley2020Engwerda, AHJSouthworth, JLebedeva, MAScanes, RJHKukura, PFletcher, SPWe report chemically fuelled out‐of‐equilibrium self‐replicating vesicles based on surfactant formation. We studied the vesicles’ autocatalytic formation using UPLC to determine monomer concentration and interferometric scattering microscopy at the nanoparticle level. Unlike related reports of chemically fuelled self‐replicating micelles, our vesicular system was too stable to surfactant degradation to be maintained out of equilibrium. The introduction of a catalyst, which introduces a second catalytic cycle into the metabolic network, was used to close the first cycle. This shows how coupled catalytic cycles can create a metabolic network that allows the creation and perseverance of fuel‐driven, out‐of‐equilibrium self‐replicating vesicles. |
| spellingShingle | Engwerda, AHJ Southworth, J Lebedeva, MA Scanes, RJH Kukura, P Fletcher, SP Coupled metabolic cycles allow out-of-equilibrium autopoietic vesicle replication |
| title | Coupled metabolic cycles allow out-of-equilibrium autopoietic vesicle replication |
| title_full | Coupled metabolic cycles allow out-of-equilibrium autopoietic vesicle replication |
| title_fullStr | Coupled metabolic cycles allow out-of-equilibrium autopoietic vesicle replication |
| title_full_unstemmed | Coupled metabolic cycles allow out-of-equilibrium autopoietic vesicle replication |
| title_short | Coupled metabolic cycles allow out-of-equilibrium autopoietic vesicle replication |
| title_sort | coupled metabolic cycles allow out of equilibrium autopoietic vesicle replication |
| work_keys_str_mv | AT engwerdaahj coupledmetaboliccyclesallowoutofequilibriumautopoieticvesiclereplication AT southworthj coupledmetaboliccyclesallowoutofequilibriumautopoieticvesiclereplication AT lebedevama coupledmetaboliccyclesallowoutofequilibriumautopoieticvesiclereplication AT scanesrjh coupledmetaboliccyclesallowoutofequilibriumautopoieticvesiclereplication AT kukurap coupledmetaboliccyclesallowoutofequilibriumautopoieticvesiclereplication AT fletchersp coupledmetaboliccyclesallowoutofequilibriumautopoieticvesiclereplication |