CTD kinase associated with yeast RNA polymerase II initiation factor b.
A kinase activity specific for the C-terminal repeat domain (CTD) of RNA polymerase II is associated with nearly homogeneous yeast general initiation factor b by three criteria: cofractionation on the basis of size and charge and coinactivation by mild heat treatment. The kinase phosphorylates the C...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1991
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| _version_ | 1826257561731465216 |
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| author | Feaver, W Gileadi, O Li, Y Kornberg, R |
| author_facet | Feaver, W Gileadi, O Li, Y Kornberg, R |
| author_sort | Feaver, W |
| collection | OXFORD |
| description | A kinase activity specific for the C-terminal repeat domain (CTD) of RNA polymerase II is associated with nearly homogeneous yeast general initiation factor b by three criteria: cofractionation on the basis of size and charge and coinactivation by mild heat treatment. The kinase phosphorylates the CTD at multiple sites in a processive manner. Factor b may possess a DNA-dependent ATPase activity as well. Both kinase and DNA-dependent ATPase activities exhibit the same nucleotide requirements as previously demonstrated for the initiation of transcription. These results support the idea that phosphorylation of the CTD lies on the pathway of transcription initiation and identify a catalytic activity of a general factor essential for the initiation process. |
| first_indexed | 2024-03-06T18:20:10Z |
| format | Journal article |
| id | oxford-uuid:0600c2cf-5b27-4cd8-a4d8-15b63fea79ad |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:20:10Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:0600c2cf-5b27-4cd8-a4d8-15b63fea79ad2022-03-26T09:00:16ZCTD kinase associated with yeast RNA polymerase II initiation factor b.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0600c2cf-5b27-4cd8-a4d8-15b63fea79adEnglishSymplectic Elements at Oxford1991Feaver, WGileadi, OLi, YKornberg, RA kinase activity specific for the C-terminal repeat domain (CTD) of RNA polymerase II is associated with nearly homogeneous yeast general initiation factor b by three criteria: cofractionation on the basis of size and charge and coinactivation by mild heat treatment. The kinase phosphorylates the CTD at multiple sites in a processive manner. Factor b may possess a DNA-dependent ATPase activity as well. Both kinase and DNA-dependent ATPase activities exhibit the same nucleotide requirements as previously demonstrated for the initiation of transcription. These results support the idea that phosphorylation of the CTD lies on the pathway of transcription initiation and identify a catalytic activity of a general factor essential for the initiation process. |
| spellingShingle | Feaver, W Gileadi, O Li, Y Kornberg, R CTD kinase associated with yeast RNA polymerase II initiation factor b. |
| title | CTD kinase associated with yeast RNA polymerase II initiation factor b. |
| title_full | CTD kinase associated with yeast RNA polymerase II initiation factor b. |
| title_fullStr | CTD kinase associated with yeast RNA polymerase II initiation factor b. |
| title_full_unstemmed | CTD kinase associated with yeast RNA polymerase II initiation factor b. |
| title_short | CTD kinase associated with yeast RNA polymerase II initiation factor b. |
| title_sort | ctd kinase associated with yeast rna polymerase ii initiation factor b |
| work_keys_str_mv | AT feaverw ctdkinaseassociatedwithyeastrnapolymeraseiiinitiationfactorb AT gileadio ctdkinaseassociatedwithyeastrnapolymeraseiiinitiationfactorb AT liy ctdkinaseassociatedwithyeastrnapolymeraseiiinitiationfactorb AT kornbergr ctdkinaseassociatedwithyeastrnapolymeraseiiinitiationfactorb |