Mechano-logical model of C. elegans germ line suggests feedback on the cell cycle
The Caenorhabditis elegans germ line is an outstanding model system in which to study the control of cell division and differentiation. Although many of the molecules that regulate germ cell proliferation and fate decisions have been identified, how these signals interact with cellular dynamics and...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Company of Biologists
2015
|
| _version_ | 1826257593818939392 |
|---|---|
| author | Atwell, K Qin, Z Gavaghan, D Kugler, H Hubbard, E Osborne, J |
| author_facet | Atwell, K Qin, Z Gavaghan, D Kugler, H Hubbard, E Osborne, J |
| author_sort | Atwell, K |
| collection | OXFORD |
| description | The Caenorhabditis elegans germ line is an outstanding model system in which to study the control of cell division and differentiation. Although many of the molecules that regulate germ cell proliferation and fate decisions have been identified, how these signals interact with cellular dynamics and physical forces within the gonad remains poorly understood. We therefore developed a dynamic, 3D in silico model of the C. elegans germ line, incorporating both the mechanical interactions between cells and the decision-making processes within cells. Our model successfully reproduces key features of the germ line during development and adulthood, including a reasonable ovulation rate, correct sperm count, and appropriate organization of the germ line into stably maintained zones. The model highlights a previously overlooked way in which germ cell pressure may influence gonadogenesis, and also predicts that adult germ cells might be subject to mechanical feedback on the cell cycle akin to contact inhibition. We provide experimental data consistent with the latter hypothesis. Finally, we present cell trajectories and ancestry recorded over the course of a simulation. The novel approaches and software described here link mechanics and cellular decision-making, and are applicable to modeling other developmental and stem cell systems. |
| first_indexed | 2024-03-06T18:20:39Z |
| format | Journal article |
| id | oxford-uuid:0628e44a-a75f-440e-a24e-4edd1d362639 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:20:39Z |
| publishDate | 2015 |
| publisher | Company of Biologists |
| record_format | dspace |
| spelling | oxford-uuid:0628e44a-a75f-440e-a24e-4edd1d3626392022-03-26T09:01:11ZMechano-logical model of C. elegans germ line suggests feedback on the cell cycleJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0628e44a-a75f-440e-a24e-4edd1d362639EnglishSymplectic Elements at OxfordCompany of Biologists2015Atwell, KQin, ZGavaghan, DKugler, HHubbard, EOsborne, JThe Caenorhabditis elegans germ line is an outstanding model system in which to study the control of cell division and differentiation. Although many of the molecules that regulate germ cell proliferation and fate decisions have been identified, how these signals interact with cellular dynamics and physical forces within the gonad remains poorly understood. We therefore developed a dynamic, 3D in silico model of the C. elegans germ line, incorporating both the mechanical interactions between cells and the decision-making processes within cells. Our model successfully reproduces key features of the germ line during development and adulthood, including a reasonable ovulation rate, correct sperm count, and appropriate organization of the germ line into stably maintained zones. The model highlights a previously overlooked way in which germ cell pressure may influence gonadogenesis, and also predicts that adult germ cells might be subject to mechanical feedback on the cell cycle akin to contact inhibition. We provide experimental data consistent with the latter hypothesis. Finally, we present cell trajectories and ancestry recorded over the course of a simulation. The novel approaches and software described here link mechanics and cellular decision-making, and are applicable to modeling other developmental and stem cell systems. |
| spellingShingle | Atwell, K Qin, Z Gavaghan, D Kugler, H Hubbard, E Osborne, J Mechano-logical model of C. elegans germ line suggests feedback on the cell cycle |
| title | Mechano-logical model of C. elegans germ line suggests feedback on the cell cycle |
| title_full | Mechano-logical model of C. elegans germ line suggests feedback on the cell cycle |
| title_fullStr | Mechano-logical model of C. elegans germ line suggests feedback on the cell cycle |
| title_full_unstemmed | Mechano-logical model of C. elegans germ line suggests feedback on the cell cycle |
| title_short | Mechano-logical model of C. elegans germ line suggests feedback on the cell cycle |
| title_sort | mechano logical model of c elegans germ line suggests feedback on the cell cycle |
| work_keys_str_mv | AT atwellk mechanologicalmodelofcelegansgermlinesuggestsfeedbackonthecellcycle AT qinz mechanologicalmodelofcelegansgermlinesuggestsfeedbackonthecellcycle AT gavaghand mechanologicalmodelofcelegansgermlinesuggestsfeedbackonthecellcycle AT kuglerh mechanologicalmodelofcelegansgermlinesuggestsfeedbackonthecellcycle AT hubbarde mechanologicalmodelofcelegansgermlinesuggestsfeedbackonthecellcycle AT osbornej mechanologicalmodelofcelegansgermlinesuggestsfeedbackonthecellcycle |