Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study
<p>Background: Dengue can cause increased vascular permeability that may lead to hypovolemic shock. Endothelial dysfunction may underlie this; however the association of endothelial nitric oxide pathways with disease severity is unknown.</p><p> Methods: We performed a prospective o...
Main Authors: | , , , , , , , , , , , , , , , |
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Format: | Journal article |
Language: | English |
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Oxford University Press
2017
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author | Yacoub, S Lam, PK Huynh, TT Ho, HH Thi, HT Van, NT Lien, LT Ha, QNT Le, DHT Mongkolspaya, J Culshaw, A Yeo, TW Wertheim, H Simmons, C Screaton, G Wills, B |
author_facet | Yacoub, S Lam, PK Huynh, TT Ho, HH Thi, HT Van, NT Lien, LT Ha, QNT Le, DHT Mongkolspaya, J Culshaw, A Yeo, TW Wertheim, H Simmons, C Screaton, G Wills, B |
author_sort | Yacoub, S |
collection | OXFORD |
description | <p>Background: Dengue can cause increased vascular permeability that may lead to hypovolemic shock. Endothelial dysfunction may underlie this; however the association of endothelial nitric oxide pathways with disease severity is unknown.</p><p> Methods: We performed a prospective observational study in two Vietnamese hospitals, assessing patients presenting early (<72 hours fever) and patients hospitalized with warning signs or severe dengue. The reactive hyperaemic index (RHI), which measures endothelium-dependent vasodilation and is a surrogate marker of endothelial function and NO bioavailability was evaluated using peripheral artery tonometry (EndoPAT) and plasma levels of L-arginine, Arginase-1 and ADMA were measured at serial time-points. The main outcome of interest was plasma leakage severity.</p><p> Results: 314 patients were enrolled, median age of the participants was 21 (IQR 13-30) years. No difference was found in the endothelial parameters between dengue and other febrile illness (OFI). Considering dengue patients, the RHI was significantly lower for patients with severe plasma leakage compared to those with no leakage (1.46 vs. 2.00, P<0.001), over acute time-points, apparent already in the early febrile phase (1.29 vs. 1.75, P=0.012). RHI correlated negatively with arginase-1, and positively with L-arginine (P=0.001). Endothelial dysfunction/NO bioavailability is associated with worse plasma leakage, occurs early in dengue illness and correlates with hypoargininaemia and high arginase-1 levels.</p> |
first_indexed | 2024-03-06T18:21:17Z |
format | Journal article |
id | oxford-uuid:06630f31-8e57-4341-9757-b757042bcc93 |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-06T18:21:17Z |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | dspace |
spelling | oxford-uuid:06630f31-8e57-4341-9757-b757042bcc932022-03-26T09:02:12ZEndothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational studyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:06630f31-8e57-4341-9757-b757042bcc93EnglishSymplectic Elements at OxfordOxford University Press2017Yacoub, SLam, PKHuynh, TTHo, HHThi, HTVan, NTLien, LTHa, QNTLe, DHTMongkolspaya, JCulshaw, AYeo, TWWertheim, HSimmons, CScreaton, GWills, B<p>Background: Dengue can cause increased vascular permeability that may lead to hypovolemic shock. Endothelial dysfunction may underlie this; however the association of endothelial nitric oxide pathways with disease severity is unknown.</p><p> Methods: We performed a prospective observational study in two Vietnamese hospitals, assessing patients presenting early (<72 hours fever) and patients hospitalized with warning signs or severe dengue. The reactive hyperaemic index (RHI), which measures endothelium-dependent vasodilation and is a surrogate marker of endothelial function and NO bioavailability was evaluated using peripheral artery tonometry (EndoPAT) and plasma levels of L-arginine, Arginase-1 and ADMA were measured at serial time-points. The main outcome of interest was plasma leakage severity.</p><p> Results: 314 patients were enrolled, median age of the participants was 21 (IQR 13-30) years. No difference was found in the endothelial parameters between dengue and other febrile illness (OFI). Considering dengue patients, the RHI was significantly lower for patients with severe plasma leakage compared to those with no leakage (1.46 vs. 2.00, P<0.001), over acute time-points, apparent already in the early febrile phase (1.29 vs. 1.75, P=0.012). RHI correlated negatively with arginase-1, and positively with L-arginine (P=0.001). Endothelial dysfunction/NO bioavailability is associated with worse plasma leakage, occurs early in dengue illness and correlates with hypoargininaemia and high arginase-1 levels.</p> |
spellingShingle | Yacoub, S Lam, PK Huynh, TT Ho, HH Thi, HT Van, NT Lien, LT Ha, QNT Le, DHT Mongkolspaya, J Culshaw, A Yeo, TW Wertheim, H Simmons, C Screaton, G Wills, B Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study |
title | Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study |
title_full | Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study |
title_fullStr | Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study |
title_full_unstemmed | Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study |
title_short | Endothelial nitric oxide pathways in the pathophysiology of dengue: a prospective observational study |
title_sort | endothelial nitric oxide pathways in the pathophysiology of dengue a prospective observational study |
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