Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction.
We examine the effects of aberrations induced by a refractive index mismatch on the signal level and resolution of single-photon (1-p) and two-photon (2-p), conventional and confocal scanning microscopes. In particular, we consider the aberrations introduced by an interface between oil/glass and wat...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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2001
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| _version_ | 1826257677965066240 |
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| author | Booth, M Wilson, T |
| author_facet | Booth, M Wilson, T |
| author_sort | Booth, M |
| collection | OXFORD |
| description | We examine the effects of aberrations induced by a refractive index mismatch on the signal level and resolution of single-photon (1-p) and two-photon (2-p), conventional and confocal scanning microscopes. In particular, we consider the aberrations introduced by an interface between oil/glass and water. Resolution is defined in terms of enclosed fluorescence, rather than full-width half-maximum, revealing more useful information for heavily aberrated point spread functions (PSFs). It is shown that, at large focusing depths, the resolution of 2-p conventional and 1-p confocal microscopes are almost identical. The benefits of aberration correction are examined by removing Zernike aberration modes. With aberration correction, the best resolution is found for 1-p confocal and 2-p confocal modes. An approximation based upon geometrical optics is also introduced which shows that the axial resolution of heavily aberrated PSFs is roughly proportional to focusing depth. |
| first_indexed | 2024-03-06T18:21:57Z |
| format | Journal article |
| id | oxford-uuid:0698d533-336c-4778-9c56-78ff159c13c8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:21:57Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:0698d533-336c-4778-9c56-78ff159c13c82022-03-26T09:03:19ZRefractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0698d533-336c-4778-9c56-78ff159c13c8EnglishSymplectic Elements at Oxford2001Booth, MWilson, TWe examine the effects of aberrations induced by a refractive index mismatch on the signal level and resolution of single-photon (1-p) and two-photon (2-p), conventional and confocal scanning microscopes. In particular, we consider the aberrations introduced by an interface between oil/glass and water. Resolution is defined in terms of enclosed fluorescence, rather than full-width half-maximum, revealing more useful information for heavily aberrated point spread functions (PSFs). It is shown that, at large focusing depths, the resolution of 2-p conventional and 1-p confocal microscopes are almost identical. The benefits of aberration correction are examined by removing Zernike aberration modes. With aberration correction, the best resolution is found for 1-p confocal and 2-p confocal modes. An approximation based upon geometrical optics is also introduced which shows that the axial resolution of heavily aberrated PSFs is roughly proportional to focusing depth. |
| spellingShingle | Booth, M Wilson, T Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction. |
| title | Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction. |
| title_full | Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction. |
| title_fullStr | Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction. |
| title_full_unstemmed | Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction. |
| title_short | Refractive-index-mismatch induced aberrations in single-photon and two-photon microscopy and the use of aberration correction. |
| title_sort | refractive index mismatch induced aberrations in single photon and two photon microscopy and the use of aberration correction |
| work_keys_str_mv | AT boothm refractiveindexmismatchinducedaberrationsinsinglephotonandtwophotonmicroscopyandtheuseofaberrationcorrection AT wilsont refractiveindexmismatchinducedaberrationsinsinglephotonandtwophotonmicroscopyandtheuseofaberrationcorrection |