The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity.
Glycosylation of macrolide antibiotics confers host cell immunity from endogenous and exogenous agents. The Streptomyces antibioticus glycosyltransferases, OleI and OleD, glycosylate and inactivate oleandomycin and diverse macrolides including erythromycin, respectively. The structure of these enzym...
| Main Authors: | , , , , , , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2007
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| _version_ | 1826257688617549824 |
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| author | Bolam, D Roberts, S Proctor, MR Turkenburg, J Dodson, E Martinez-Fleites, C Yang, M Davis, B Davies, G Gilbert, H |
| author_facet | Bolam, D Roberts, S Proctor, MR Turkenburg, J Dodson, E Martinez-Fleites, C Yang, M Davis, B Davies, G Gilbert, H |
| author_sort | Bolam, D |
| collection | OXFORD |
| description | Glycosylation of macrolide antibiotics confers host cell immunity from endogenous and exogenous agents. The Streptomyces antibioticus glycosyltransferases, OleI and OleD, glycosylate and inactivate oleandomycin and diverse macrolides including erythromycin, respectively. The structure of these enzyme-ligand complexes, in tandem with kinetic analysis of site-directed variants, provide insight into the interaction of macrolides with their synthetic apparatus. Erythromycin binds to OleD and the 23S RNA of its target ribosome in the same conformation and, although the antibiotic contains a large number of polar groups, its interaction with these macromolecules is primarily through hydrophobic contacts. Erythromycin and oleandomycin, when bound to OleD and OleI, respectively, adopt different conformations, reflecting a subtle effect on sugar positioning by virtue of a single change in the macrolide backbone. The data reported here provide structural insight into the mechanism of resistance to both endogenous and exogenous antibiotics, and will provide a platform for the future redesign of these catalysts for antibiotic remodelling. |
| first_indexed | 2024-03-06T18:22:07Z |
| format | Journal article |
| id | oxford-uuid:06a89c31-2b42-44c4-9b6e-f29af8f58ea5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:22:07Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:06a89c31-2b42-44c4-9b6e-f29af8f58ea52022-03-26T09:03:42ZThe crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:06a89c31-2b42-44c4-9b6e-f29af8f58ea5EnglishSymplectic Elements at Oxford2007Bolam, DRoberts, SProctor, MRTurkenburg, JDodson, EMartinez-Fleites, CYang, MDavis, BDavies, GGilbert, HGlycosylation of macrolide antibiotics confers host cell immunity from endogenous and exogenous agents. The Streptomyces antibioticus glycosyltransferases, OleI and OleD, glycosylate and inactivate oleandomycin and diverse macrolides including erythromycin, respectively. The structure of these enzyme-ligand complexes, in tandem with kinetic analysis of site-directed variants, provide insight into the interaction of macrolides with their synthetic apparatus. Erythromycin binds to OleD and the 23S RNA of its target ribosome in the same conformation and, although the antibiotic contains a large number of polar groups, its interaction with these macromolecules is primarily through hydrophobic contacts. Erythromycin and oleandomycin, when bound to OleD and OleI, respectively, adopt different conformations, reflecting a subtle effect on sugar positioning by virtue of a single change in the macrolide backbone. The data reported here provide structural insight into the mechanism of resistance to both endogenous and exogenous antibiotics, and will provide a platform for the future redesign of these catalysts for antibiotic remodelling. |
| spellingShingle | Bolam, D Roberts, S Proctor, MR Turkenburg, J Dodson, E Martinez-Fleites, C Yang, M Davis, B Davies, G Gilbert, H The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity. |
| title | The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity. |
| title_full | The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity. |
| title_fullStr | The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity. |
| title_full_unstemmed | The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity. |
| title_short | The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity. |
| title_sort | crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity |
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