The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis.
Delta-like 4 (DLL4), a membrane-bound ligand belonging to the Notch signaling family, plays a fundamental role in vascular development and angiogenesis. We identified a conserved microRNA family, miR-30, which targets DLL4. Overexpression of miR-30b in endothelial cells led to increased vessel numbe...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2012
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| _version_ | 1797051631973433344 |
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| author | Bridge, G Monteiro, R Henderson, S Emuss, V Lagos, D Georgopoulou, D Patient, R Boshoff, C |
| author_facet | Bridge, G Monteiro, R Henderson, S Emuss, V Lagos, D Georgopoulou, D Patient, R Boshoff, C |
| author_sort | Bridge, G |
| collection | OXFORD |
| description | Delta-like 4 (DLL4), a membrane-bound ligand belonging to the Notch signaling family, plays a fundamental role in vascular development and angiogenesis. We identified a conserved microRNA family, miR-30, which targets DLL4. Overexpression of miR-30b in endothelial cells led to increased vessel number and length in an in vitro model of sprouting angiogenesis. Microinjection of miR-30 mimics into zebrafish embryos resulted in suppression of dll4 and subsequent excessive sprouting of intersegmental vessels and reduction in dorsal aorta diameter. Use of a target protector against the miR-30 site within the dll4 3'UTR up-regulated dll4 and synergized with Vegfa signaling knockdown to inhibit angiogenesis. Furthermore, restoration of miR-30b or miR-30c expression during Kaposi sarcoma herpesvirus (KSHV) infection attenuated viral induction of DLL4. Together these results demonstrate that the highly conserved molecular targeting of DLL4 by the miR-30 family regulates angiogenesis. |
| first_indexed | 2024-03-06T18:22:18Z |
| format | Journal article |
| id | oxford-uuid:06b714c2-c110-4902-a826-1d1f13e2267c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:22:18Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:06b714c2-c110-4902-a826-1d1f13e2267c2022-03-26T09:03:57ZThe microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:06b714c2-c110-4902-a826-1d1f13e2267cEnglishSymplectic Elements at Oxford2012Bridge, GMonteiro, RHenderson, SEmuss, VLagos, DGeorgopoulou, DPatient, RBoshoff, CDelta-like 4 (DLL4), a membrane-bound ligand belonging to the Notch signaling family, plays a fundamental role in vascular development and angiogenesis. We identified a conserved microRNA family, miR-30, which targets DLL4. Overexpression of miR-30b in endothelial cells led to increased vessel number and length in an in vitro model of sprouting angiogenesis. Microinjection of miR-30 mimics into zebrafish embryos resulted in suppression of dll4 and subsequent excessive sprouting of intersegmental vessels and reduction in dorsal aorta diameter. Use of a target protector against the miR-30 site within the dll4 3'UTR up-regulated dll4 and synergized with Vegfa signaling knockdown to inhibit angiogenesis. Furthermore, restoration of miR-30b or miR-30c expression during Kaposi sarcoma herpesvirus (KSHV) infection attenuated viral induction of DLL4. Together these results demonstrate that the highly conserved molecular targeting of DLL4 by the miR-30 family regulates angiogenesis. |
| spellingShingle | Bridge, G Monteiro, R Henderson, S Emuss, V Lagos, D Georgopoulou, D Patient, R Boshoff, C The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. |
| title | The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. |
| title_full | The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. |
| title_fullStr | The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. |
| title_full_unstemmed | The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. |
| title_short | The microRNA-30 family targets DLL4 to modulate endothelial cell behavior during angiogenesis. |
| title_sort | microrna 30 family targets dll4 to modulate endothelial cell behavior during angiogenesis |
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