Comparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reaction.

Comparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reaction.

We compare the accuracy of a variety of Fluorescence Fluctuation Spectroscopy (FFS) methods for the study of Förster Resonance Energy Transfer (FRET) assays. As an example, the cleavage of a doubly labeled, FRET-active peptide substrate by the protease Trypsin is monitored and analyzed using methods...

Полное описание

Библиографические подробности
Главные авторы:	Eggeling, C, Jäger, S, Winkler, D, Kask, P
Формат:	Journal article
Язык:	English
Опубликовано:	2005

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