Comparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reaction.

Comparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reaction.

We compare the accuracy of a variety of Fluorescence Fluctuation Spectroscopy (FFS) methods for the study of Förster Resonance Energy Transfer (FRET) assays. As an example, the cleavage of a doubly labeled, FRET-active peptide substrate by the protease Trypsin is monitored and analyzed using methods...

书目详细资料
Main Authors:	Eggeling, C, Jäger, S, Winkler, D, Kask, P
格式:	Journal article
语言:	English
出版:	2005

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