High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine.
<h4>Background</h4> <p>Neisseria meningitidis is a globally important cause of meningitis and septicaemia. Twelve capsular groups of meningococci are known, and quadrivalent vaccines against four of these (A, C, W and Y) are available as plain-polysaccharide and protein-polysaccha...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
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BioMed Central
2017
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| _version_ | 1797051692344147968 |
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| author | O'Connor, D Clutterbuck, E Thompson, A Snape, M Ramasamy, M Kelly, D Pollard, A |
| author_facet | O'Connor, D Clutterbuck, E Thompson, A Snape, M Ramasamy, M Kelly, D Pollard, A |
| author_sort | O'Connor, D |
| collection | OXFORD |
| description | <h4>Background</h4> <p>Neisseria meningitidis is a globally important cause of meningitis and septicaemia. Twelve capsular groups of meningococci are known, and quadrivalent vaccines against four of these (A, C, W and Y) are available as plain-polysaccharide and protein-polysaccharide conjugate vaccines. Here we apply contemporary methods to describe B-cell responses to meningococcal polysaccharide and conjugate vaccines.</p> <h4>Methods</h4> <p>Twenty adults were randomly assigned to receive either a meningococcal plain-polysaccharide or conjugate vaccine; one month later all received the conjugate vaccine. Blood samples were taken pre-vaccination and 7, 21 and 28 days after vaccination; B-cell responses were assessed by ELISpot, serum bactericidal assay, flow cytometry and gene expression microarray.</p> <h4>Results</h4> <p>Seven days after an initial dose of either vaccine, a gene expression signature characteristic of plasmablasts was detectable. The frequency of newly generated plasma cells (CXCR3+HLA-DR+) and the expression of transcripts derived from IGKC and IGHG2 correlated with immunogenicity. Notably, using an independent dataset, the expression of glucosamine (N-acetyl)-6-sulfatase was found to reproducibly correlate with the magnitude of immune response. Transcriptomic and flow cytometric data revealed depletion of switched memory B cells following plain-polysaccharide vaccine.</p> <h4>Conclusions</h4> <p>These data describe distinct gene signatures associated with the production of high-avidity antibody and a plain-polysaccharide-specific signature, possibly linked to polysaccharide-induced hyporesponsiveness.</p> |
| first_indexed | 2024-03-06T18:23:08Z |
| format | Journal article |
| id | oxford-uuid:070022b5-4ccc-4764-98ee-a944bc66010a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:23:08Z |
| publishDate | 2017 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | oxford-uuid:070022b5-4ccc-4764-98ee-a944bc66010a2022-03-26T09:05:23ZHigh-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:070022b5-4ccc-4764-98ee-a944bc66010aEnglishSymplectic Elements at OxfordBioMed Central2017O'Connor, DClutterbuck, EThompson, ASnape, MRamasamy, MKelly, DPollard, A <h4>Background</h4> <p>Neisseria meningitidis is a globally important cause of meningitis and septicaemia. Twelve capsular groups of meningococci are known, and quadrivalent vaccines against four of these (A, C, W and Y) are available as plain-polysaccharide and protein-polysaccharide conjugate vaccines. Here we apply contemporary methods to describe B-cell responses to meningococcal polysaccharide and conjugate vaccines.</p> <h4>Methods</h4> <p>Twenty adults were randomly assigned to receive either a meningococcal plain-polysaccharide or conjugate vaccine; one month later all received the conjugate vaccine. Blood samples were taken pre-vaccination and 7, 21 and 28 days after vaccination; B-cell responses were assessed by ELISpot, serum bactericidal assay, flow cytometry and gene expression microarray.</p> <h4>Results</h4> <p>Seven days after an initial dose of either vaccine, a gene expression signature characteristic of plasmablasts was detectable. The frequency of newly generated plasma cells (CXCR3+HLA-DR+) and the expression of transcripts derived from IGKC and IGHG2 correlated with immunogenicity. Notably, using an independent dataset, the expression of glucosamine (N-acetyl)-6-sulfatase was found to reproducibly correlate with the magnitude of immune response. Transcriptomic and flow cytometric data revealed depletion of switched memory B cells following plain-polysaccharide vaccine.</p> <h4>Conclusions</h4> <p>These data describe distinct gene signatures associated with the production of high-avidity antibody and a plain-polysaccharide-specific signature, possibly linked to polysaccharide-induced hyporesponsiveness.</p> |
| spellingShingle | O'Connor, D Clutterbuck, E Thompson, A Snape, M Ramasamy, M Kelly, D Pollard, A High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. |
| title | High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. |
| title_full | High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. |
| title_fullStr | High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. |
| title_full_unstemmed | High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. |
| title_short | High-dimensional assessment of B-cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine. |
| title_sort | high dimensional assessment of b cell responses to quadrivalent meningococcal conjugate and plain polysaccharide vaccine |
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