Characterization of aortic root atherosclerosis in ApoE knockout mice: high-resolution in vivo and ex vivo MRM with histological correlation.
In vivo, cardiac-gated, black-blood, and ex vivo magnetic resonance microscopy (MRM) images of the aortic root, and histopathology data were obtained from 12 transgenic and wild-type (WT) mice. MRM was performed using a black-blood imaging spin-echo sequence with upstream and downstream in-flow satu...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1826257786872266752 |
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| author | Itskovich, V Choudhury, R Aguinaldo, J Fallon, J Omerhodzic, S Fisher, E Fayad, Z |
| author_facet | Itskovich, V Choudhury, R Aguinaldo, J Fallon, J Omerhodzic, S Fisher, E Fayad, Z |
| author_sort | Itskovich, V |
| collection | OXFORD |
| description | In vivo, cardiac-gated, black-blood, and ex vivo magnetic resonance microscopy (MRM) images of the aortic root, and histopathology data were obtained from 12 transgenic and wild-type (WT) mice. MRM was performed using a black-blood imaging spin-echo sequence with upstream and downstream in-flow saturation pulses to obtain aortic root images in three contrast techniques: proton density-weighted (PDW), T(1)- (T(1)W), and T(2)-weighted (T(2)W). Aortic wall thickness and area were measured and correlated with histopathology data (R > 0.90). Ex vivo lesion components (lipid core, fibrous tissue, and cell tissue) were identified and characterized by differing image contrast in PDW, T(1)W, and T(2)W MRM, and by histopathology. The differences between WT and transgenic mice for maximal wall thickness and area were statistically significant (P < 0.05). This study demonstrates the feasibility of in vivo murine aortic root lesion assessment and ex vivo plaque characterization by MRM. |
| first_indexed | 2024-03-06T18:23:42Z |
| format | Journal article |
| id | oxford-uuid:07332833-c0c9-4596-99bf-3a1fbe3180b8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:23:42Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:07332833-c0c9-4596-99bf-3a1fbe3180b82022-03-26T09:06:23ZCharacterization of aortic root atherosclerosis in ApoE knockout mice: high-resolution in vivo and ex vivo MRM with histological correlation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:07332833-c0c9-4596-99bf-3a1fbe3180b8EnglishSymplectic Elements at Oxford2003Itskovich, VChoudhury, RAguinaldo, JFallon, JOmerhodzic, SFisher, EFayad, ZIn vivo, cardiac-gated, black-blood, and ex vivo magnetic resonance microscopy (MRM) images of the aortic root, and histopathology data were obtained from 12 transgenic and wild-type (WT) mice. MRM was performed using a black-blood imaging spin-echo sequence with upstream and downstream in-flow saturation pulses to obtain aortic root images in three contrast techniques: proton density-weighted (PDW), T(1)- (T(1)W), and T(2)-weighted (T(2)W). Aortic wall thickness and area were measured and correlated with histopathology data (R > 0.90). Ex vivo lesion components (lipid core, fibrous tissue, and cell tissue) were identified and characterized by differing image contrast in PDW, T(1)W, and T(2)W MRM, and by histopathology. The differences between WT and transgenic mice for maximal wall thickness and area were statistically significant (P < 0.05). This study demonstrates the feasibility of in vivo murine aortic root lesion assessment and ex vivo plaque characterization by MRM. |
| spellingShingle | Itskovich, V Choudhury, R Aguinaldo, J Fallon, J Omerhodzic, S Fisher, E Fayad, Z Characterization of aortic root atherosclerosis in ApoE knockout mice: high-resolution in vivo and ex vivo MRM with histological correlation. |
| title | Characterization of aortic root atherosclerosis in ApoE knockout mice: high-resolution in vivo and ex vivo MRM with histological correlation. |
| title_full | Characterization of aortic root atherosclerosis in ApoE knockout mice: high-resolution in vivo and ex vivo MRM with histological correlation. |
| title_fullStr | Characterization of aortic root atherosclerosis in ApoE knockout mice: high-resolution in vivo and ex vivo MRM with histological correlation. |
| title_full_unstemmed | Characterization of aortic root atherosclerosis in ApoE knockout mice: high-resolution in vivo and ex vivo MRM with histological correlation. |
| title_short | Characterization of aortic root atherosclerosis in ApoE knockout mice: high-resolution in vivo and ex vivo MRM with histological correlation. |
| title_sort | characterization of aortic root atherosclerosis in apoe knockout mice high resolution in vivo and ex vivo mrm with histological correlation |
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