Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria.
IgG and IgG3 antibodies to merozoite surface protein-2 (MSP-2) of Plasmodium falciparum have been associated with protection from clinical malaria in independent studies. We determined whether this protection was allele-specific by testing whether children who developed clinical malaria lacked IgG/I...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2010
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| _version_ | 1826257813956984832 |
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| author | Osier, F Murungi, L Fegan, G Tuju, J Tetteh, K Bull, P Conway, D Marsh, K |
| author_facet | Osier, F Murungi, L Fegan, G Tuju, J Tetteh, K Bull, P Conway, D Marsh, K |
| author_sort | Osier, F |
| collection | OXFORD |
| description | IgG and IgG3 antibodies to merozoite surface protein-2 (MSP-2) of Plasmodium falciparum have been associated with protection from clinical malaria in independent studies. We determined whether this protection was allele-specific by testing whether children who developed clinical malaria lacked IgG/IgG3 antibodies specific to the dominant msp2 parasite genotypes detected during clinical episodes. We analysed pre-existing IgG and IgG1/IgG3 antibodies to antigens representing the major dimorphic types of MSP-2 by ELISA. We used quantitative real-time PCR to determine the dominant msp2 alleles in parasites detected in clinical episodes. Over half (55%, 80/146) of infections contained both allelic types. Single or dominant IC1- and FC27-like alleles were detected in 46% and 42% of infections respectively, and both types were equally dominant in 12%. High levels of IgG/IgG3 antibodies to the FC27-like antigen were not significantly associated with a lower likelihood of clinical episodes caused by parasites bearing FC27-like compared to IC1-like alleles, and vice versa for IgG/IgG3 antibodies to the IC1-like antigen. These findings were supported by competition ELISAs which demonstrated the presence of IgG antibodies to allele-specific epitopes within both antigens. Thus, even for this well-studied antigen, the importance of an allele-specific component of naturally acquired protective immunity to malaria remains to be confirmed. |
| first_indexed | 2024-03-06T18:24:07Z |
| format | Journal article |
| id | oxford-uuid:075700ba-f9f1-455d-b59c-9bc19173f316 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:24:07Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:075700ba-f9f1-455d-b59c-9bc19173f3162022-03-26T09:07:05ZAllele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:075700ba-f9f1-455d-b59c-9bc19173f316EnglishSymplectic Elements at Oxford2010Osier, FMurungi, LFegan, GTuju, JTetteh, KBull, PConway, DMarsh, KIgG and IgG3 antibodies to merozoite surface protein-2 (MSP-2) of Plasmodium falciparum have been associated with protection from clinical malaria in independent studies. We determined whether this protection was allele-specific by testing whether children who developed clinical malaria lacked IgG/IgG3 antibodies specific to the dominant msp2 parasite genotypes detected during clinical episodes. We analysed pre-existing IgG and IgG1/IgG3 antibodies to antigens representing the major dimorphic types of MSP-2 by ELISA. We used quantitative real-time PCR to determine the dominant msp2 alleles in parasites detected in clinical episodes. Over half (55%, 80/146) of infections contained both allelic types. Single or dominant IC1- and FC27-like alleles were detected in 46% and 42% of infections respectively, and both types were equally dominant in 12%. High levels of IgG/IgG3 antibodies to the FC27-like antigen were not significantly associated with a lower likelihood of clinical episodes caused by parasites bearing FC27-like compared to IC1-like alleles, and vice versa for IgG/IgG3 antibodies to the IC1-like antigen. These findings were supported by competition ELISAs which demonstrated the presence of IgG antibodies to allele-specific epitopes within both antigens. Thus, even for this well-studied antigen, the importance of an allele-specific component of naturally acquired protective immunity to malaria remains to be confirmed. |
| spellingShingle | Osier, F Murungi, L Fegan, G Tuju, J Tetteh, K Bull, P Conway, D Marsh, K Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria. |
| title | Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria. |
| title_full | Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria. |
| title_fullStr | Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria. |
| title_full_unstemmed | Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria. |
| title_short | Allele-specific antibodies to Plasmodium falciparum merozoite surface protein-2 and protection against clinical malaria. |
| title_sort | allele specific antibodies to plasmodium falciparum merozoite surface protein 2 and protection against clinical malaria |
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