Cytosolic [Ca2+] regulation of InsP3-evoked puffs.
InsP3-mediated puffs are fundamental building blocks of cellular Ca2+ signalling, and arise through the concerted opening of clustered InsP3Rs (InsP3 receptors) co-ordinated via Ca2+-induced Ca2+ release. Although the Ca2+ dependency of InsP3Rs has been extensively studied at the single channel leve...
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| Format: | Journal article |
| Language: | English |
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2013
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| _version_ | 1826257816919212032 |
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| author | Yamasaki-Mann, M Demuro, A Parker, I |
| author_facet | Yamasaki-Mann, M Demuro, A Parker, I |
| author_sort | Yamasaki-Mann, M |
| collection | OXFORD |
| description | InsP3-mediated puffs are fundamental building blocks of cellular Ca2+ signalling, and arise through the concerted opening of clustered InsP3Rs (InsP3 receptors) co-ordinated via Ca2+-induced Ca2+ release. Although the Ca2+ dependency of InsP3Rs has been extensively studied at the single channel level, little is known as to how changes in basal cytosolic [Ca2+] would alter the dynamics of InsP3-evoked Ca2+ signals in intact cells. To explore this question, we expressed Ca2+-permeable channels (nicotinic acetylcholine receptors) in the plasma membrane of voltage-clamped Xenopus oocytes to regulate cytosolic [Ca2+] by changing the electrochemical gradient for extracellular Ca2+ entry, and imaged Ca2+ liberation evoked by photolysis of caged InsP3. Elevation of basal cytosolic [Ca2+] strongly increased the amplitude and shortened the latency of global Ca2+ waves. In oocytes loaded with EGTA to localize Ca2+ signals, the number of sites at which puffs were observed and the frequency and latency of puffs were strongly dependent on cytosolic [Ca2+], whereas puff amplitudes were only weakly affected. The results of the present study indicate that basal cytosolic [Ca2+] strongly affects the triggering of puffs, but has less of an effect on puffs once they have been initiated. |
| first_indexed | 2024-03-06T18:24:10Z |
| format | Journal article |
| id | oxford-uuid:075cf567-7692-4ee8-a859-6826ac895ed0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:24:10Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:075cf567-7692-4ee8-a859-6826ac895ed02022-03-26T09:07:05ZCytosolic [Ca2+] regulation of InsP3-evoked puffs.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:075cf567-7692-4ee8-a859-6826ac895ed0EnglishSymplectic Elements at Oxford2013Yamasaki-Mann, MDemuro, AParker, IInsP3-mediated puffs are fundamental building blocks of cellular Ca2+ signalling, and arise through the concerted opening of clustered InsP3Rs (InsP3 receptors) co-ordinated via Ca2+-induced Ca2+ release. Although the Ca2+ dependency of InsP3Rs has been extensively studied at the single channel level, little is known as to how changes in basal cytosolic [Ca2+] would alter the dynamics of InsP3-evoked Ca2+ signals in intact cells. To explore this question, we expressed Ca2+-permeable channels (nicotinic acetylcholine receptors) in the plasma membrane of voltage-clamped Xenopus oocytes to regulate cytosolic [Ca2+] by changing the electrochemical gradient for extracellular Ca2+ entry, and imaged Ca2+ liberation evoked by photolysis of caged InsP3. Elevation of basal cytosolic [Ca2+] strongly increased the amplitude and shortened the latency of global Ca2+ waves. In oocytes loaded with EGTA to localize Ca2+ signals, the number of sites at which puffs were observed and the frequency and latency of puffs were strongly dependent on cytosolic [Ca2+], whereas puff amplitudes were only weakly affected. The results of the present study indicate that basal cytosolic [Ca2+] strongly affects the triggering of puffs, but has less of an effect on puffs once they have been initiated. |
| spellingShingle | Yamasaki-Mann, M Demuro, A Parker, I Cytosolic [Ca2+] regulation of InsP3-evoked puffs. |
| title | Cytosolic [Ca2+] regulation of InsP3-evoked puffs. |
| title_full | Cytosolic [Ca2+] regulation of InsP3-evoked puffs. |
| title_fullStr | Cytosolic [Ca2+] regulation of InsP3-evoked puffs. |
| title_full_unstemmed | Cytosolic [Ca2+] regulation of InsP3-evoked puffs. |
| title_short | Cytosolic [Ca2+] regulation of InsP3-evoked puffs. |
| title_sort | cytosolic ca2 regulation of insp3 evoked puffs |
| work_keys_str_mv | AT yamasakimannm cytosolicca2regulationofinsp3evokedpuffs AT demuroa cytosolicca2regulationofinsp3evokedpuffs AT parkeri cytosolicca2regulationofinsp3evokedpuffs |