Preclinical comparison of novel rotator cuff repair scaffolds
<p><b>Background:</b> Rotator cuff tears affect up to 16% of the adult population, but inadequate treatment options are currently available. Our research group has developed a range of degradable scaffolds, manufactured from electrospinning and weaving, designed to assist the tend...
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| Format: | Thesis |
| Language: | English |
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2014
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| _version_ | 1797051847419101184 |
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| author | Lostis, E |
| author2 | Carr, A |
| author_facet | Carr, A Lostis, E |
| author_sort | Lostis, E |
| collection | OXFORD |
| description | <p><b>Background:</b> Rotator cuff tears affect up to 16% of the adult population, but inadequate treatment options are currently available. Our research group has developed a range of degradable scaffolds, manufactured from electrospinning and weaving, designed to assist the tendon repair both biologically and mechanically. The scaffolds have been successfully tested <em>in vitro</em>, however, to further assess the potential of these materials for clinical applications, an <em>in vivo</em> study is necessary.</p> <p><b>Aim:</b> The overall aim of this study was to assess the safety and biocompatibility of the scaffolds <em>in vivo</em>. The objectives included optimisation of the weaving process and assessment of the systemic and local host reaction to scaffolds upon implantation.</p> <p><b>Methodology:</b> The weaving process was first optimised to achieve satisfactory mechanical properties for the woven components. For the <em>in vivo</em> study, four different scaffolds were implanted into the shoulder of Lewis rats. The infraspinatus was transected and repaired using the scaffolds and at six timepoints up to 5 months, the shoulder joint was dissected for histological assessment. The foreign body response was assessed by hematoxylin and eosin (H&E) staining and by evaluating the presence of foreign body giant cells (FBGCs).</p> <p><b>Results and Discussion:</b> The optimisation of the weaving process resulted in the development of scaffolds with improved tensile strength. Upon implantation, most scaffolds did not indicate any severe adverse reaction. However histology revealed that the presence of large quantities of electrospun material elicits a strong foreign body reaction, with significantly more FBGCs in the group which had an electrospun scaffold compared to the positive control.</p> <p><b>Conclusion:</b> Scaffolds containing no or moderate electrospun materials seemed to be well tolerated by the rats and appear safe for human use. However, implanting large volumes of electrospun material may elicit an unwanted foreign body reaction and more <em>in vivo</em> work is needed to understand its tissue integration.</p> |
| first_indexed | 2024-03-06T18:25:12Z |
| format | Thesis |
| id | oxford-uuid:07b673a5-4329-4da1-b34e-74ee39e4c43a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:25:12Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:07b673a5-4329-4da1-b34e-74ee39e4c43a2022-03-26T09:09:01ZPreclinical comparison of novel rotator cuff repair scaffoldsThesishttp://purl.org/coar/resource_type/c_bdccuuid:07b673a5-4329-4da1-b34e-74ee39e4c43aMedical SciencesOrthopaedicsEnglishOxford University Research Archive - Valet2014Lostis, ECarr, AMouthuy, P <p><b>Background:</b> Rotator cuff tears affect up to 16% of the adult population, but inadequate treatment options are currently available. Our research group has developed a range of degradable scaffolds, manufactured from electrospinning and weaving, designed to assist the tendon repair both biologically and mechanically. The scaffolds have been successfully tested <em>in vitro</em>, however, to further assess the potential of these materials for clinical applications, an <em>in vivo</em> study is necessary.</p> <p><b>Aim:</b> The overall aim of this study was to assess the safety and biocompatibility of the scaffolds <em>in vivo</em>. The objectives included optimisation of the weaving process and assessment of the systemic and local host reaction to scaffolds upon implantation.</p> <p><b>Methodology:</b> The weaving process was first optimised to achieve satisfactory mechanical properties for the woven components. For the <em>in vivo</em> study, four different scaffolds were implanted into the shoulder of Lewis rats. The infraspinatus was transected and repaired using the scaffolds and at six timepoints up to 5 months, the shoulder joint was dissected for histological assessment. The foreign body response was assessed by hematoxylin and eosin (H&E) staining and by evaluating the presence of foreign body giant cells (FBGCs).</p> <p><b>Results and Discussion:</b> The optimisation of the weaving process resulted in the development of scaffolds with improved tensile strength. Upon implantation, most scaffolds did not indicate any severe adverse reaction. However histology revealed that the presence of large quantities of electrospun material elicits a strong foreign body reaction, with significantly more FBGCs in the group which had an electrospun scaffold compared to the positive control.</p> <p><b>Conclusion:</b> Scaffolds containing no or moderate electrospun materials seemed to be well tolerated by the rats and appear safe for human use. However, implanting large volumes of electrospun material may elicit an unwanted foreign body reaction and more <em>in vivo</em> work is needed to understand its tissue integration.</p> |
| spellingShingle | Medical Sciences Orthopaedics Lostis, E Preclinical comparison of novel rotator cuff repair scaffolds |
| title | Preclinical comparison of novel rotator cuff repair scaffolds |
| title_full | Preclinical comparison of novel rotator cuff repair scaffolds |
| title_fullStr | Preclinical comparison of novel rotator cuff repair scaffolds |
| title_full_unstemmed | Preclinical comparison of novel rotator cuff repair scaffolds |
| title_short | Preclinical comparison of novel rotator cuff repair scaffolds |
| title_sort | preclinical comparison of novel rotator cuff repair scaffolds |
| topic | Medical Sciences Orthopaedics |
| work_keys_str_mv | AT lostise preclinicalcomparisonofnovelrotatorcuffrepairscaffolds |