Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei
Trypanosoma brucei is a uni-cellular protist that causes African sleeping sickness. These parasites have a flagellum that is attached to the cell body and is indispensible for its motility. The flagellum consists of a canonical 9+2 axoneme and a paraflagellar rod (PFR), an intricate tripartite, fibr...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2012
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| _version_ | 1826258043696840704 |
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| author | Höög, J Bouchet-Marquis, C McIntosh, JR Hoenger, A Gull, K |
| author_facet | Höög, J Bouchet-Marquis, C McIntosh, JR Hoenger, A Gull, K |
| author_sort | Höög, J |
| collection | OXFORD |
| description | Trypanosoma brucei is a uni-cellular protist that causes African sleeping sickness. These parasites have a flagellum that is attached to the cell body and is indispensible for its motility. The flagellum consists of a canonical 9+2 axoneme and a paraflagellar rod (PFR), an intricate tripartite, fibrous structure that is connected to the axoneme. In this paper we describe results from cryo-electron tomography of unperturbed flagella. This method revealed novel structures that are likely involved in attaching the flagellum to the cell. We also show the first cryo-electron tomographic images of a basal body in situ, revealing electron dense structures inside its triplet microtubules. Sub-tomogram averaging of the PFR revealed that its distal region is organized as an orthorhombic crystal. |
| first_indexed | 2024-03-06T18:27:47Z |
| format | Journal article |
| id | oxford-uuid:089477e7-3ed5-42b3-8ec7-977af31128ac |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:27:47Z |
| publishDate | 2012 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:089477e7-3ed5-42b3-8ec7-977af31128ac2022-03-26T09:13:39ZCryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma bruceiJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:089477e7-3ed5-42b3-8ec7-977af31128acEnglishSymplectic Elements at OxfordElsevier2012Höög, JBouchet-Marquis, CMcIntosh, JRHoenger, AGull, KTrypanosoma brucei is a uni-cellular protist that causes African sleeping sickness. These parasites have a flagellum that is attached to the cell body and is indispensible for its motility. The flagellum consists of a canonical 9+2 axoneme and a paraflagellar rod (PFR), an intricate tripartite, fibrous structure that is connected to the axoneme. In this paper we describe results from cryo-electron tomography of unperturbed flagella. This method revealed novel structures that are likely involved in attaching the flagellum to the cell. We also show the first cryo-electron tomographic images of a basal body in situ, revealing electron dense structures inside its triplet microtubules. Sub-tomogram averaging of the PFR revealed that its distal region is organized as an orthorhombic crystal. |
| spellingShingle | Höög, J Bouchet-Marquis, C McIntosh, JR Hoenger, A Gull, K Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei |
| title | Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei |
| title_full | Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei |
| title_fullStr | Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei |
| title_full_unstemmed | Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei |
| title_short | Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei |
| title_sort | cryo electron tomography and 3 d analysis of the intact flagellum in trypanosoma brucei |
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