Global contraction or local growth, bleb shape depends on more than just cell structure.
When the plasma membrane of a cell locally delaminates from its actin cortex the membrane is pushed outwards due to the cell׳s internal fluid pressure. The resulting spherical protrusion is known as a bleb. A cell׳s ability to function correctly is highly dependent on the production of such protrusi...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2015
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| _version_ | 1797052201737125888 |
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| author | Woolley, T Gaffney, E Oliver, J Waters, S Baker, R Goriely, A |
| author_facet | Woolley, T Gaffney, E Oliver, J Waters, S Baker, R Goriely, A |
| author_sort | Woolley, T |
| collection | OXFORD |
| description | When the plasma membrane of a cell locally delaminates from its actin cortex the membrane is pushed outwards due to the cell׳s internal fluid pressure. The resulting spherical protrusion is known as a bleb. A cell׳s ability to function correctly is highly dependent on the production of such protrusions with the correct size and shape. Here, we investigate the nucleation of large blebs from small, local neck regions. A mathematical model of a cell׳s membrane, cortex and interconnecting adhesions demonstrates that these three components are unable to capture experimentally observed bleb shapes without the addition of further assumptions. We have identified that combinations of global cortex contraction and localised membrane growth are the most promising methods for generating prototypical blebs. Currently, neither proposed mechanism has been fully tested experimentally and, thus, we propose experiments that will distinguish between the two methods of bleb production. |
| first_indexed | 2024-03-06T18:29:08Z |
| format | Journal article |
| id | oxford-uuid:09082130-4e96-4931-aabd-9256d9ced2da |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:29:08Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:09082130-4e96-4931-aabd-9256d9ced2da2022-03-26T09:16:04ZGlobal contraction or local growth, bleb shape depends on more than just cell structure.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:09082130-4e96-4931-aabd-9256d9ced2daEnglishSymplectic Elements at OxfordElsevier2015Woolley, TGaffney, EOliver, JWaters, SBaker, RGoriely, AWhen the plasma membrane of a cell locally delaminates from its actin cortex the membrane is pushed outwards due to the cell׳s internal fluid pressure. The resulting spherical protrusion is known as a bleb. A cell׳s ability to function correctly is highly dependent on the production of such protrusions with the correct size and shape. Here, we investigate the nucleation of large blebs from small, local neck regions. A mathematical model of a cell׳s membrane, cortex and interconnecting adhesions demonstrates that these three components are unable to capture experimentally observed bleb shapes without the addition of further assumptions. We have identified that combinations of global cortex contraction and localised membrane growth are the most promising methods for generating prototypical blebs. Currently, neither proposed mechanism has been fully tested experimentally and, thus, we propose experiments that will distinguish between the two methods of bleb production. |
| spellingShingle | Woolley, T Gaffney, E Oliver, J Waters, S Baker, R Goriely, A Global contraction or local growth, bleb shape depends on more than just cell structure. |
| title | Global contraction or local growth, bleb shape depends on more than just cell structure. |
| title_full | Global contraction or local growth, bleb shape depends on more than just cell structure. |
| title_fullStr | Global contraction or local growth, bleb shape depends on more than just cell structure. |
| title_full_unstemmed | Global contraction or local growth, bleb shape depends on more than just cell structure. |
| title_short | Global contraction or local growth, bleb shape depends on more than just cell structure. |
| title_sort | global contraction or local growth bleb shape depends on more than just cell structure |
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