A novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs).
The self-assembly properties of a protein encoded by the yeast retrotransposon Ty can be exploited to produce large amounts of recombinant, particulate fusion proteins as hybrid Ty virus-like particles (Ty-VLPs). This system has now been adapted to allow the release of the additional protein by inco...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
1989
|
| _version_ | 1797052454278266880 |
|---|---|
| author | Gilmour, J Senior, J Burns, N Esnouf, M Gull, K Kingsman, S Kingsman, A Adams, SE |
| author_facet | Gilmour, J Senior, J Burns, N Esnouf, M Gull, K Kingsman, S Kingsman, A Adams, SE |
| author_sort | Gilmour, J |
| collection | OXFORD |
| description | The self-assembly properties of a protein encoded by the yeast retrotransposon Ty can be exploited to produce large amounts of recombinant, particulate fusion proteins as hybrid Ty virus-like particles (Ty-VLPs). This system has now been adapted to allow the release of the additional protein by incorporation of a protease cleavage site between the yeast carrier protein and the protein of interest. The purification of the additional protein is facilitated by exploiting the ease with which Ty-VLPs can be purified from other yeast cell components due to their particulate nature. We have used this modified system to produce hybrid particles containing the HIV-1 p24 protein downstream of the recognition sequence for the blood coagulation factor Xa. The p24 was released from the particles by proteolytic cleavage and rapidly separated from the residual particulate material using centrifugation and standard chromatography techniques. This procedure has been used to purify milligram quantities of HIV-1 p24 protein that reacts with anti-p24 sera and elicits the production of p24-specific antibodies in experimental animals. |
| first_indexed | 2024-03-06T18:31:48Z |
| format | Journal article |
| id | oxford-uuid:09e4ed4a-b52f-4e3d-baac-3b01e7a89f78 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:31:48Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:09e4ed4a-b52f-4e3d-baac-3b01e7a89f782022-03-26T09:20:53ZA novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs).Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:09e4ed4a-b52f-4e3d-baac-3b01e7a89f78EnglishSymplectic Elements at Oxford1989Gilmour, JSenior, JBurns, NEsnouf, MGull, KKingsman, SKingsman, AAdams, SEThe self-assembly properties of a protein encoded by the yeast retrotransposon Ty can be exploited to produce large amounts of recombinant, particulate fusion proteins as hybrid Ty virus-like particles (Ty-VLPs). This system has now been adapted to allow the release of the additional protein by incorporation of a protease cleavage site between the yeast carrier protein and the protein of interest. The purification of the additional protein is facilitated by exploiting the ease with which Ty-VLPs can be purified from other yeast cell components due to their particulate nature. We have used this modified system to produce hybrid particles containing the HIV-1 p24 protein downstream of the recognition sequence for the blood coagulation factor Xa. The p24 was released from the particles by proteolytic cleavage and rapidly separated from the residual particulate material using centrifugation and standard chromatography techniques. This procedure has been used to purify milligram quantities of HIV-1 p24 protein that reacts with anti-p24 sera and elicits the production of p24-specific antibodies in experimental animals. |
| spellingShingle | Gilmour, J Senior, J Burns, N Esnouf, M Gull, K Kingsman, S Kingsman, A Adams, SE A novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs). |
| title | A novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs). |
| title_full | A novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs). |
| title_fullStr | A novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs). |
| title_full_unstemmed | A novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs). |
| title_short | A novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs). |
| title_sort | novel method for the purification of hiv 1 p24 protein from hybrid ty virus like particles ty vlps |
| work_keys_str_mv | AT gilmourj anovelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT seniorj anovelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT burnsn anovelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT esnoufm anovelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT gullk anovelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT kingsmans anovelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT kingsmana anovelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT adamsse anovelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT gilmourj novelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT seniorj novelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT burnsn novelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT esnoufm novelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT gullk novelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT kingsmans novelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT kingsmana novelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps AT adamsse novelmethodforthepurificationofhiv1p24proteinfromhybridtyviruslikeparticlestyvlps |