The acidic macromolecules in rabbit cortical bone tissue.
Rabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one...
Main Authors: | , , |
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Format: | Journal article |
Language: | English |
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1982
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author | Diamond, A Triffitt, J Herring, G |
author_facet | Diamond, A Triffitt, J Herring, G |
author_sort | Diamond, A |
collection | OXFORD |
description | Rabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one containing chondroitin sulphate and the other containing material resembling keratan sulphate mixed with a smaller amount of chondroitin sulphate. Sialoglycoproteins were detected by specific chemical introduction of tritium label into the sialic acid residues of the acidic glycoprotein fraction. Following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography, the presence of numerous distinct sialoglycoprotein components was demonstrated. |
first_indexed | 2024-03-06T18:32:27Z |
format | Journal article |
id | oxford-uuid:0a223bc4-2b19-436d-ae96-3d2538f28fed |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-06T18:32:27Z |
publishDate | 1982 |
record_format | dspace |
spelling | oxford-uuid:0a223bc4-2b19-436d-ae96-3d2538f28fed2022-03-26T09:22:07ZThe acidic macromolecules in rabbit cortical bone tissue.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0a223bc4-2b19-436d-ae96-3d2538f28fedEnglishSymplectic Elements at Oxford1982Diamond, ATriffitt, JHerring, GRabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one containing chondroitin sulphate and the other containing material resembling keratan sulphate mixed with a smaller amount of chondroitin sulphate. Sialoglycoproteins were detected by specific chemical introduction of tritium label into the sialic acid residues of the acidic glycoprotein fraction. Following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography, the presence of numerous distinct sialoglycoprotein components was demonstrated. |
spellingShingle | Diamond, A Triffitt, J Herring, G The acidic macromolecules in rabbit cortical bone tissue. |
title | The acidic macromolecules in rabbit cortical bone tissue. |
title_full | The acidic macromolecules in rabbit cortical bone tissue. |
title_fullStr | The acidic macromolecules in rabbit cortical bone tissue. |
title_full_unstemmed | The acidic macromolecules in rabbit cortical bone tissue. |
title_short | The acidic macromolecules in rabbit cortical bone tissue. |
title_sort | acidic macromolecules in rabbit cortical bone tissue |
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