Interferometric scattering microscopy
Interferometric scattering microscopy (iSCAT) is an extremely sensitive imaging method based on the efficient detection of light scattered by nanoscopic objects. The ability to, at least in principle, maintain high imaging contrast independent of the exposure time or the scattering cross section of...
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| Format: | Journal article |
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Annual Reviews
2019
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| _version_ | 1797052515192143872 |
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| author | Young, G Kukura, P |
| author_facet | Young, G Kukura, P |
| author_sort | Young, G |
| collection | OXFORD |
| description | Interferometric scattering microscopy (iSCAT) is an extremely sensitive imaging method based on the efficient detection of light scattered by nanoscopic objects. The ability to, at least in principle, maintain high imaging contrast independent of the exposure time or the scattering cross section of the object allows for unique applications in single-particle tracking, label-free imaging of nanoscopic (dis)assembly, and quantitative single-molecule characterization. We illustrate these capabilities in areas as diverse as mechanistic studies of motor protein function, viral capsid assembly, and single-molecule mass measurement in solution. We anticipate that iSCAT will become a widely used approach to unravel previously hidden details of biomolecular dynamics and interactions. |
| first_indexed | 2024-03-06T18:32:39Z |
| format | Journal article |
| id | oxford-uuid:0a318d07-5a69-49df-964a-e2e45748ce59 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T18:32:39Z |
| publishDate | 2019 |
| publisher | Annual Reviews |
| record_format | dspace |
| spelling | oxford-uuid:0a318d07-5a69-49df-964a-e2e45748ce592022-03-26T09:22:28ZInterferometric scattering microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0a318d07-5a69-49df-964a-e2e45748ce59Symplectic Elements at OxfordAnnual Reviews2019Young, GKukura, PInterferometric scattering microscopy (iSCAT) is an extremely sensitive imaging method based on the efficient detection of light scattered by nanoscopic objects. The ability to, at least in principle, maintain high imaging contrast independent of the exposure time or the scattering cross section of the object allows for unique applications in single-particle tracking, label-free imaging of nanoscopic (dis)assembly, and quantitative single-molecule characterization. We illustrate these capabilities in areas as diverse as mechanistic studies of motor protein function, viral capsid assembly, and single-molecule mass measurement in solution. We anticipate that iSCAT will become a widely used approach to unravel previously hidden details of biomolecular dynamics and interactions. |
| spellingShingle | Young, G Kukura, P Interferometric scattering microscopy |
| title | Interferometric scattering microscopy |
| title_full | Interferometric scattering microscopy |
| title_fullStr | Interferometric scattering microscopy |
| title_full_unstemmed | Interferometric scattering microscopy |
| title_short | Interferometric scattering microscopy |
| title_sort | interferometric scattering microscopy |
| work_keys_str_mv | AT youngg interferometricscatteringmicroscopy AT kukurap interferometricscatteringmicroscopy |