rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome.
We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousand...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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Nature Publishing Group
2016
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| _version_ | 1826258414670446592 |
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| author | Kwok, C Marsico, G Sahakyan, A Chambers, V Balasubramanian, S |
| author_facet | Kwok, C Marsico, G Sahakyan, A Chambers, V Balasubramanian, S |
| author_sort | Kwok, C |
| collection | OXFORD |
| description | We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability. |
| first_indexed | 2024-03-06T18:33:37Z |
| format | Journal article |
| id | oxford-uuid:0a7ec504-3650-4c6b-831f-73ccffdf60a4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:33:37Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:0a7ec504-3650-4c6b-831f-73ccffdf60a42022-03-26T09:24:09ZrG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0a7ec504-3650-4c6b-831f-73ccffdf60a4EnglishSymplectic Elements at OxfordNature Publishing Group2016Kwok, CMarsico, GSahakyan, AChambers, VBalasubramanian, SWe introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability. |
| spellingShingle | Kwok, C Marsico, G Sahakyan, A Chambers, V Balasubramanian, S rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. |
| title | rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. |
| title_full | rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. |
| title_fullStr | rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. |
| title_full_unstemmed | rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. |
| title_short | rG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. |
| title_sort | rg4 seq reveals widespread formation of g quadruplex structures in the human transcriptome |
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