Rhizobium determinants of rhizosphere persistence and root colonization
Bacterial persistence in the rhizosphere and colonization of root niches are critical for the establishment of many beneficial plant–bacteria interactions including those between Rhizobium leguminosarum and its host legumes. Despite this, most studies on R. leguminosarum have focused on its symbioti...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Sprog: | English |
| Udgivet: |
Oxford University Press
2024
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| _version_ | 1826314783738036224 |
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| author | Knights, HE Ramachandran, VK Jorrin, B Ledermann, R Parsons, JD Aroney, STN Poole, PS |
| author_facet | Knights, HE Ramachandran, VK Jorrin, B Ledermann, R Parsons, JD Aroney, STN Poole, PS |
| author_sort | Knights, HE |
| collection | OXFORD |
| description | Bacterial persistence in the rhizosphere and colonization of root niches are critical for the establishment of many beneficial plant–bacteria interactions including those between Rhizobium leguminosarum and its host legumes. Despite this, most studies on R. leguminosarum have focused on its symbiotic lifestyle as an endosymbiont in root nodules. Here, we use random barcode transposon sequencing to assay gene contributions of R. leguminosarum during competitive growth in the rhizosphere and colonization of various plant species. This facilitated the identification of 189 genes commonly required for growth in diverse plant rhizospheres, mutation of 111 of which also affected subsequent root colonization (rhizosphere progressive), and a further 119 genes necessary for colonization. Common determinants reveal a need to synthesize essential compounds (amino acids, ribonucleotides, and cofactors), adapt metabolic function, respond to external stimuli, and withstand various stresses (such as changes in osmolarity). Additionally, chemotaxis and flagella-mediated motility are prerequisites for root colonization. Many genes showed plant-specific dependencies highlighting significant adaptation to different plant species. This work provides a greater understanding of factors promoting rhizosphere fitness and root colonization in plant-beneficial bacteria, facilitating their exploitation for agricultural benefit. |
| first_indexed | 2024-09-25T04:16:27Z |
| format | Journal article |
| id | oxford-uuid:0a997d48-ff9b-4174-9fcb-0287cc4c5347 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:13:07Z |
| publishDate | 2024 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:0a997d48-ff9b-4174-9fcb-0287cc4c53472024-10-16T09:11:48ZRhizobium determinants of rhizosphere persistence and root colonizationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0a997d48-ff9b-4174-9fcb-0287cc4c5347EnglishJisc Publications RouterOxford University Press2024Knights, HERamachandran, VKJorrin, BLedermann, RParsons, JDAroney, STNPoole, PSBacterial persistence in the rhizosphere and colonization of root niches are critical for the establishment of many beneficial plant–bacteria interactions including those between Rhizobium leguminosarum and its host legumes. Despite this, most studies on R. leguminosarum have focused on its symbiotic lifestyle as an endosymbiont in root nodules. Here, we use random barcode transposon sequencing to assay gene contributions of R. leguminosarum during competitive growth in the rhizosphere and colonization of various plant species. This facilitated the identification of 189 genes commonly required for growth in diverse plant rhizospheres, mutation of 111 of which also affected subsequent root colonization (rhizosphere progressive), and a further 119 genes necessary for colonization. Common determinants reveal a need to synthesize essential compounds (amino acids, ribonucleotides, and cofactors), adapt metabolic function, respond to external stimuli, and withstand various stresses (such as changes in osmolarity). Additionally, chemotaxis and flagella-mediated motility are prerequisites for root colonization. Many genes showed plant-specific dependencies highlighting significant adaptation to different plant species. This work provides a greater understanding of factors promoting rhizosphere fitness and root colonization in plant-beneficial bacteria, facilitating their exploitation for agricultural benefit. |
| spellingShingle | Knights, HE Ramachandran, VK Jorrin, B Ledermann, R Parsons, JD Aroney, STN Poole, PS Rhizobium determinants of rhizosphere persistence and root colonization |
| title | Rhizobium determinants of rhizosphere persistence and root colonization |
| title_full | Rhizobium determinants of rhizosphere persistence and root colonization |
| title_fullStr | Rhizobium determinants of rhizosphere persistence and root colonization |
| title_full_unstemmed | Rhizobium determinants of rhizosphere persistence and root colonization |
| title_short | Rhizobium determinants of rhizosphere persistence and root colonization |
| title_sort | rhizobium determinants of rhizosphere persistence and root colonization |
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