Plasmacytoid dendritic cells prime IFN-gamma-secreting melanoma-specific CD8 lymphocytes and are found in primary melanoma lesions.
Plasmacytoid dendritic cells (PDC) are a small population of leukocytes specialized in the production of type I IFN. It has been shown that PDC have a potent T cell stimulatory capacity in allogeneic mixed lymphocyte reaction, However, their role in initiating primary immune responses remains elusiv...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1797052761404080128 |
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| author | Salio, M Cella, M Vermi, W Facchetti, F Palmowski, M Smith, C Shepherd, D Colonna, M Cerundolo, V |
| author_facet | Salio, M Cella, M Vermi, W Facchetti, F Palmowski, M Smith, C Shepherd, D Colonna, M Cerundolo, V |
| author_sort | Salio, M |
| collection | OXFORD |
| description | Plasmacytoid dendritic cells (PDC) are a small population of leukocytes specialized in the production of type I IFN. It has been shown that PDC have a potent T cell stimulatory capacity in allogeneic mixed lymphocyte reaction, However, their role in initiating primary immune responses remains elusive. We report that blood PDC efficiently prime naive CD8(+) lymphocytes specific for the melan-A(26-35) epitope to become IFN-gamma producing cells in vitro. In addition, we found that CD40L-stimulated PDC induce expression on primed melan-A-specific T cells of cutaneous lymphocyte antigen and L-selectin (CD62L), homing receptors that allow the migration of effector cells to the inflamed skin. Finally, we show that PDC can be found in the peri-tumoral area of most primary cutaneous melanomas in vivo and that type I IFN-containing supernatants derived from PDC increase melanoma cell surface expression of CD95 and MHC class I and class II molecules in vitro. Our results suggest a new immunomodulatory role for tissue infiltrating PDC, which may prime tumor-specific T cell responses and affect tumor growth via soluble factors. |
| first_indexed | 2024-03-06T18:35:12Z |
| format | Journal article |
| id | oxford-uuid:0afa519a-1d9f-4b24-a76a-16b330ee47c5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T18:35:12Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:0afa519a-1d9f-4b24-a76a-16b330ee47c52022-03-26T09:27:02ZPlasmacytoid dendritic cells prime IFN-gamma-secreting melanoma-specific CD8 lymphocytes and are found in primary melanoma lesions.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:0afa519a-1d9f-4b24-a76a-16b330ee47c5EnglishSymplectic Elements at Oxford2003Salio, MCella, MVermi, WFacchetti, FPalmowski, MSmith, CShepherd, DColonna, MCerundolo, VPlasmacytoid dendritic cells (PDC) are a small population of leukocytes specialized in the production of type I IFN. It has been shown that PDC have a potent T cell stimulatory capacity in allogeneic mixed lymphocyte reaction, However, their role in initiating primary immune responses remains elusive. We report that blood PDC efficiently prime naive CD8(+) lymphocytes specific for the melan-A(26-35) epitope to become IFN-gamma producing cells in vitro. In addition, we found that CD40L-stimulated PDC induce expression on primed melan-A-specific T cells of cutaneous lymphocyte antigen and L-selectin (CD62L), homing receptors that allow the migration of effector cells to the inflamed skin. Finally, we show that PDC can be found in the peri-tumoral area of most primary cutaneous melanomas in vivo and that type I IFN-containing supernatants derived from PDC increase melanoma cell surface expression of CD95 and MHC class I and class II molecules in vitro. Our results suggest a new immunomodulatory role for tissue infiltrating PDC, which may prime tumor-specific T cell responses and affect tumor growth via soluble factors. |
| spellingShingle | Salio, M Cella, M Vermi, W Facchetti, F Palmowski, M Smith, C Shepherd, D Colonna, M Cerundolo, V Plasmacytoid dendritic cells prime IFN-gamma-secreting melanoma-specific CD8 lymphocytes and are found in primary melanoma lesions. |
| title | Plasmacytoid dendritic cells prime IFN-gamma-secreting melanoma-specific CD8 lymphocytes and are found in primary melanoma lesions. |
| title_full | Plasmacytoid dendritic cells prime IFN-gamma-secreting melanoma-specific CD8 lymphocytes and are found in primary melanoma lesions. |
| title_fullStr | Plasmacytoid dendritic cells prime IFN-gamma-secreting melanoma-specific CD8 lymphocytes and are found in primary melanoma lesions. |
| title_full_unstemmed | Plasmacytoid dendritic cells prime IFN-gamma-secreting melanoma-specific CD8 lymphocytes and are found in primary melanoma lesions. |
| title_short | Plasmacytoid dendritic cells prime IFN-gamma-secreting melanoma-specific CD8 lymphocytes and are found in primary melanoma lesions. |
| title_sort | plasmacytoid dendritic cells prime ifn gamma secreting melanoma specific cd8 lymphocytes and are found in primary melanoma lesions |
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