Applications of droplet interface bilayers: specific capacitance measurements and membrane protein corralling

<p>Droplet Interface Bilayers (DIBs) have a number of attributes that distinguish them from conventional artificial lipid bilayers. In particular, the ability to manipulate bilayers mechanically is explored in this thesis. Directed bilayer area changes are used to make precise measurements of...

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Main Authors: Gross, L, Dr Linda C. M. Gross
Other Authors: Wallace, M
Format: Thesis
Language:English
Published: 2011
Subjects:
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author Gross, L
Dr Linda C. M. Gross
author2 Wallace, M
author_facet Wallace, M
Gross, L
Dr Linda C. M. Gross
author_sort Gross, L
collection OXFORD
description <p>Droplet Interface Bilayers (DIBs) have a number of attributes that distinguish them from conventional artificial lipid bilayers. In particular, the ability to manipulate bilayers mechanically is explored in this thesis. Directed bilayer area changes are used to make precise measurements of the specific capacitance of DIBs and to control the two dimensional concentration of a membrane protein reconstituted in the bilayer.</p><p> Chapter 1 provides a general introduction to the role of the lipid membrane en- vironment in the function of biological membranes and their integral proteins. An overview of model lipid bilayer systems is given.</p><p> Chapter 2 introduces work carried out in this laboratory previously and illustrates the experimental setup of DIBs. Some important bilayer biophysical concepts are covered to provide the theoretical background to experiments in this and in later chapters. Results from the characterisation of DIBs are reported, and an account of the development of methods to manipulate the bilayer by mechanical means is given.</p><p> Chapter 3 describes experiments that apply bilayer area manipulation in DIBs to achieve precise measurement of specific capacitance in a range of lipid systems.</p><p> Chapter 4 reports results from experiments investigating the response of bilayer specific capacitance to an applied potential.</p><p> Chapter 5 covers the background and experimental setup for total internal fluo- rescence microscopy experiments in DIBs and describes the expression, purification and characterisation of the bacterial β-barrel membrane protein pore α-Hemolysin.</p><p> Chapter 6 describes experiments that apply the mechanical manipulation of bilayer area in DIBs to the corralling and control of the surface density of α-Hemolysin.</p>
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spelling oxford-uuid:0b7ffba6-b86d-499c-a93f-3b2fc46a427b2022-03-26T09:29:51ZApplications of droplet interface bilayers: specific capacitance measurements and membrane protein corrallingThesishttp://purl.org/coar/resource_type/c_db06uuid:0b7ffba6-b86d-499c-a93f-3b2fc46a427bPhysical & theoretical chemistryBiophysical chemistryBiophysicsLife SciencesEnglishOxford University Research Archive - Valet2011Gross, LDr Linda C. M. GrossWallace, M<p>Droplet Interface Bilayers (DIBs) have a number of attributes that distinguish them from conventional artificial lipid bilayers. In particular, the ability to manipulate bilayers mechanically is explored in this thesis. Directed bilayer area changes are used to make precise measurements of the specific capacitance of DIBs and to control the two dimensional concentration of a membrane protein reconstituted in the bilayer.</p><p> Chapter 1 provides a general introduction to the role of the lipid membrane en- vironment in the function of biological membranes and their integral proteins. An overview of model lipid bilayer systems is given.</p><p> Chapter 2 introduces work carried out in this laboratory previously and illustrates the experimental setup of DIBs. Some important bilayer biophysical concepts are covered to provide the theoretical background to experiments in this and in later chapters. Results from the characterisation of DIBs are reported, and an account of the development of methods to manipulate the bilayer by mechanical means is given.</p><p> Chapter 3 describes experiments that apply bilayer area manipulation in DIBs to achieve precise measurement of specific capacitance in a range of lipid systems.</p><p> Chapter 4 reports results from experiments investigating the response of bilayer specific capacitance to an applied potential.</p><p> Chapter 5 covers the background and experimental setup for total internal fluo- rescence microscopy experiments in DIBs and describes the expression, purification and characterisation of the bacterial β-barrel membrane protein pore α-Hemolysin.</p><p> Chapter 6 describes experiments that apply the mechanical manipulation of bilayer area in DIBs to the corralling and control of the surface density of α-Hemolysin.</p>
spellingShingle Physical & theoretical chemistry
Biophysical chemistry
Biophysics
Life Sciences
Gross, L
Dr Linda C. M. Gross
Applications of droplet interface bilayers: specific capacitance measurements and membrane protein corralling
title Applications of droplet interface bilayers: specific capacitance measurements and membrane protein corralling
title_full Applications of droplet interface bilayers: specific capacitance measurements and membrane protein corralling
title_fullStr Applications of droplet interface bilayers: specific capacitance measurements and membrane protein corralling
title_full_unstemmed Applications of droplet interface bilayers: specific capacitance measurements and membrane protein corralling
title_short Applications of droplet interface bilayers: specific capacitance measurements and membrane protein corralling
title_sort applications of droplet interface bilayers specific capacitance measurements and membrane protein corralling
topic Physical & theoretical chemistry
Biophysical chemistry
Biophysics
Life Sciences
work_keys_str_mv AT grossl applicationsofdropletinterfacebilayersspecificcapacitancemeasurementsandmembraneproteincorralling
AT drlindacmgross applicationsofdropletinterfacebilayersspecificcapacitancemeasurementsandmembraneproteincorralling