Rescue of synthetic RNAs into thogoto and influenza A virus particles using core proteins purified from Thogoto virus.
The ribonucleoprotein (RNP) complexes of Thogoto virus (THOV), a tick-borne orthomyxovirus, have been purified from detergent-lysed virions. The purified RNPs were then disrupted by centrifugation through a CsCl-glycerol gradient to obtain fractions highly enriched in nucleoprotein (NP) and virtuall...
Main Authors: | Gómez-Puertas, P, Leahy, M, Nuttall, P, Portela, A |
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Format: | Journal article |
Language: | English |
Published: |
2000
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